姜黄油的抗炎镇痛及体外抗氧化活性-食品科学.pdf

※营养卫生 食品科学 2018, Vol.39, No.01 243 姜黄油的抗炎镇痛及体外抗氧化活性 卢彩会，牟德华* （河北科技大学生物科学与工程学院，河北石家庄 050000 ） 摘  要：目的：探讨姜黄油的抗炎、镇痛及体外抗氧化作用。方法：采用二甲苯致小鼠耳肿胀并用角叉菜胶致小鼠 足肿胀，综合评价姜黄油的抗炎效果；对小鼠连续灌胃给药后，观察热板痛阈值及冰醋酸致小鼠的扭体次数来评价 其镇痛效果；通过测定清除1,1-二苯基-2-三硝基苯肼自由基、超氧阴离子自由基，总抗氧化能力及对油脂的保护能 力，评价姜黄油及姜黄油胃肠消化液的体外抗氧化效果。结果表明：不同剂量的姜黄油表现出不同程度的抗炎、镇 痛效果；同时姜黄油及姜黄油的胃肠消化液均具有良好的抗氧化效果，并且与剂量成依赖关系。姜黄油总的抗氧化 / 效果最好，其半最大效应质量浓度为0.75 mg mL ，但经胃肠液消化后的姜黄油的抗氧化效果要高于姜黄油。 关键词：姜黄油；抗炎；镇痛；体外抗氧化 Antiinflammatory, Analgesic and in Vitro Antioxidant Activities of Turmeric Oil * LU Caihui, MOU Dehua (College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050000, China) Abstract: Objective: To investigate the antiinflammatory, analgesic and in vitro antioxidant effects of turmeric oil. Methods: Xylene-induced mouse ear swelling and carrageenan-induced mouse paw swelling tests were used to comprehensively evaluate the antiinflammatory effect. The analgesic effect of continuous intragastric administration of turmeric oil was evaluated by hot plate pain threshold and the number of writhing responses induced by acetic acid. The in vitro antioxidant activity was investigated by determining the scavenging capacity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion free radicals, total antioxidant capacity and inhibitory effect on lipid peroxidation. Results: Turmeric oil at different doses showed different antiinflammatory and analgesic effects. At the same time, both gastrointestinally digested and native turmeric oil had good antioxidant e