茶树花转录组微卫星分布特征.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2014, 40(1): 80−85 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2014.00080 茶树花转录组微卫星分布特征 王丽鸳 韦 康 张成才 成 浩* / , 310008 : Perl , SSR , SSR 10 290 , 12 582 SSR, 2.41 kb SSR340 , (AG/CT)n (44.99%)49 586 Unigene , 10 490 SSR , 1917 , 0.102 SSR/1000 bp, 3.072 SSR/1000 bp (1140, 59.5%), (524, 27.3%) 20 bp , 20 bp 25.2% , , : ; ; ; Characterization of Micorsatellites in Tea (Camellia sinensis) Floral Transcriptome * WANG Li-Yuan, WEI Kang, ZHANG Cheng-Cai, and CHENG Hao Tea Research Institute, Chinese Academy of Agricultural Sciences / National Center for Tea Improvement, Hangzhou 310008, China Abstract: The microsatellites or simple sequence repeats (SSRs) in Camellia sinensis floral transcriptome were characterized. A total of 12 582 SSRs were identified in 10 290 unigenes, with one SSR per 2.41 kb. Among all 340 SSR motifs, (AG/CT) was the n most frequent repeat motif (44.99%). A total of 10 409 SSRs occurred in 49586 unigenes with Blast matches to annotated proteins in four databases, only 1917 of which occurred in protein-coding regions of these sequences. The density of SSRs was much higher in non-coding regions than in coding regions (0.102 SSRs per 1000 base pairs in coding regions vs. 3.072 in non-coding regions). In the six repeat motifs, tri-nucleotide repeats were the most abundant in coding regions (1140), followed by hexa-nucleotide (524) repeats.