皮肤中miRNA研究综述ppt课件.ppt

microRNAs the DNA hidden treasure Guo sen 2012/2/28 A Simplified History of Life on Earth Ratio of Non-Protein-Coding DNA to Total Genomic DNA During Evolution non-protein-coding RNAs represent a fundamental advance in the genetic operating system of higher organisms Non-protein-coding RNA rRNA tRNA small nuclear(sn) RNA small nucleolar (sno) RNAs micro (mi) RNAs some of the lesser-known RNAs such as vault RNAs, rasi-RNAs and piRNAs Discovery of MiRNA Lee, R. C., Feinbaum, R. L. Ambros, V. Cell 75, 843–854 (1993). Wightman, B., Ha, I. Ruvkun, G. Cell 75, 855–862 (1993). What is MiRNA miRNAs are non-coding, single-stranded RNAs of ~22 nucleotides miRNAs are a large family of gene regulators miRNAs suppress the expression of their target genes by suppressing the translation of target mRNAs Where is MiRNA How does MiRNA Work miRNAs Inhibit Translation by Inducing Ribosome Drop-Off Role of miRNA in gene regulation miRNAs usually bind to their targets with incomplete complementarity a single miRNA might bind to as many as 200 gene targets and that these targets can be diverse in their function miRNAs potentially control the expression of about one-third of human mRNAs and have a broad influence over several diverse genetic pathways MiRNAs and Skin disease Phenotypic and morphological alterations accompanied in Dicer1 conditional null skin. miRNAs and skin morphogenesis The skin-speci?c miR-203 was recently linked to skin Morphogenesis. miR-203 represses p63 expression, an important factor in epidermal cell proliferation and differentiation miRNAs and wound healing Inflammatory:miR-140 has been shown to have a modulating effect on zebrafish platelet-derived growth factor(PDGF) receptor a miRNAs and psoriasis miR-203 was the ?rst miRNA found to be signi?cantly overexpressed in psoriasis patients Other miRNAs linked to psoriasis progression are miR-146a and miR-125b, which indirectly or directly regulate TNF-a expression miRNAs and Melanoma miR-