The Nuclear Receptor Nr5a2 Can Replace Oct4 in the Reprogramming of Murine Somatic Cells to Pluripotent Cells英文文献.pdfVIP

Cell Stem Cell Short Article The Nuclear Receptor Nr5a2 Can Replace Oct4 in the Reprogramming of Murine Somatic Cells to Pluripotent Cells Jian-Chien Dominic Heng,1,4,7 Bo Feng,1,7 Jianyong Han,2,7 Jianming Jiang,1 Petra Kraus,2 Jia-Hui Ng,1,4 Yuriy L. Orlov,3 Mikael Huss,3 Lin Yang,1 Thomas Lufkin,2,5 Bing Lim,2,6 and Huck-Hui Ng1,4,5,* 1Gene Regulation Laboratory 2Stem Cell and Developmental Biology 3Computational and Systems Biology Group Genome Institute of Singapore, Singapore 138672, Singapore 4NUS Graduate School for Integrative Sciences and Engineering, Singapore 117597, Singapore 5Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore 6Center for Life Sciences, Harvard Medical School, 330 Brookline Avenue, Boston, MA 02115, USA 7These authors contributed equally to this work *Correspondence: nghh@gis.a-star.edu.sg DOI 10.1016/j.stem.2009.12.009 SUMMARY ages and a diverse range of species have been successfully reprogrammed (Feng et al., 2009b). Besides the four reprogram- Somatic cells can be reprogrammed to induced ming factors discovered in the groundbreaking study of Yama- pluripotent stem cells (iPSCs) with the introduction naka, other factors such as NANOG and LIN28 were also found of Oct4, Sox2, Klf4, and c-Myc. Among these four to participate in reprogramming (Yu et al., 2007). Recently, Esrrb, factors, Oct4 is critical in inducing pluripotency an orphan nuclear receptor, was shown to be a reprogramming because no transcription factor can substitute for factor capable of replacing Klf4 in the reprogramming of MEFs Oct4, whereas Sox2, Klf4, and c-Myc can be re- (Feng et al., 2009a). C