Membrane Stretching Machine：拉伸膜机.ppt

Mark Bradford Kevin Feeley Tony Martinelli Jeff Snyder Jacob Stephens Our sponsor sought a device capable of recreating in vivo conditions on cell cultures. This device applies the mechanical stresses and strains that a particular type of cell culture would encounter within the body. There is a device available commercially for this purpose; however, it is prohibitively expensive. The purpose of this project was to design an alternative that is less expensive, thus enabling more researchers access to this type of machine. Must be considerably less expensive to manufacture Must use standard well plates Must be computer controlled Must fit in an incubator Must tolerate humidity up to 100% Must be easy to use Must allow easy access to well plate Must measure forces applied to cultures Must measure displacement applied to cultures Must have high repeatability Several concepts were brainstormed All concepts accomplish same functions but with different components Those components included the clipping mechanism, driver, and type of well plate Figure 1: Clipping Mechanism Concept One of these concepts is sketched below Figure 2: Concept 2 The clips secure the membrane to the stretch plate The version shown below was determined to be the most user-friendly, while accomplishing their primary function Figure 3: Solid Model of the Clip The stretch plate transfers force from the driver to the membranes The final version is designed for standard 8-well plates Figure 4: Solid Model of Stretch Plates The base constrains the stretch plate and holds the well plate in place It was also designed to allow easy removal of the well plate Figure 5: Base with mounting sub-assembly The driver in the final design was picked to be a linear actuator The driver provides force to stretch the membrane Figure 6: RRA-23 Linear Actuator Two sensors were needed, one that measured force and the other to measure displacement The force sensor was a Load Cell strain gage, and the displacement senso