荧光定量原理与应用第二版.pptVIP

* Comparative Ct methods can be broken down into two categories. Normalized Expression and Relative Quantity Relative quantity is calculated by the equation E^delta Ct where delta Ct is the difference between a chosen sample and any given sample in the experiment. This information is available in the iQ5 online help as well as some documentation supplied in training. The value is a non normalized value. No correction for loading or cell number is present in the equation. The researcher typically takes great care to ensure s/he is loading exactly the same amount of nucleic acid for each