读书报告6BT棉花对根际土壤酶活性和土壤微生物的影响演示课件.pptVIP

Effect of Bt Cotton on Enzymes Activity and Microorganisms in Rhizosphere ;Outline;1 Introduction;Although there are diverse benefits of Bt cotton, public concern also exist because both in vitro and in vivo studies on Bt cotton showed that Bt toxin produced in leaves, stems and roots of Bt cotton plants is introduced in soil.;Bt toxin released in soil get adsorbed or bound on clay particles, humic components, or organic mineral complexes and then be protected against degradation by soil microorganisms (Tapp et al., 1995) .;Some studies indicate that Bt cotton has no negative effects on soil flora and fauna and may even have beneficial effects (Saxena and Stotzky 2001; Sarkar et al., 2009)， while some have reported adverse effects (Cui and Xia 2000; Tan et al.,2002).; 2.2 Soil Sampling Soil samples from the rhizosphere of Bt cotton and non Bt cotton plants were collected at regular interval (30days interval, coinciding with different growth stage of cotton) since the date of sowings till harvesting in all the three years. Bt棉花和非Bt棉花根际土壤样品在整个三年内都是自播种之日起有规律间隔采集的(30天间隔,正好与棉花不同生育期相符合)。 ;2.3 Dehydrogenase 、Alkaline phosphatase 、Urease 、Nitrate reductase activity;2.4 Nematodes（线虫）、Collembola（弹尾目） and Ants;3. Results and discussion;On average dehydrogenase, alkaline phosphatase, nitrate reductaase and urease enzymes activity was high in Bt cotton rhizosphere as compared to non Bt rhizosphere.;Figure 1. Variations in activity of (a) Dehydrogenase (b) Alkaline phosphatase (c) Nitrate reductase and (d)Urease enzymes at different growth stages in Bt and Non Bt cotton rhizosphere;Except dehydrogenase enzyme, differences in activity of alkaline phosphatase, nitrate reductase and urease enzymes between Bt and non Bt plants rhizosphere were statistically non significant. Over the crop growth period (of 150 days) the activity of Phosphatase, nitrate reductaase and urease enzymes activity was high in the rhizosphere at early vegetative and flowering stage,whereas ac