元素的形态分析3.pptVIP

（3）反相液相色谱（RP-LC） RP-LC是基于待测物在非极性的固定相和极性的流动相之间的分配实现分离，它也是HPLC中的一种最为常见的分离模式，特别在分离不同极性的有机化合物中有着广泛的应用。 它有两种分离模式： A、直接分离模式 一般而言，一些中性的金属有机化合物最适合用反相色谱法直接分离。如硒氨基酸、有机钴、有机锰以及有机铅化合物等。 B、柱前衍生模式 为了能用反相色谱法分离一些极性的金属无机或有机化合物，可以在柱分离前，用适当的化学方法对试样中的待分离形态进行衍生化预处理，使之转化为中性或憎水的金属络合物，从而实现分离。 例. Speciation of Methylcyclopentadienyl Manganese Tricarbonyl by High-Performance Liquid Chromatography-Diode Laser Atomic Absorption Spectrometry（David J. Butcher, et al., Anal. Chem. 1999, 71: 5379） Methylcyclopentadienyl manganese tricarbonyl (MMT) is a fuel additive that has been marketed for use in unleaded gasoline since December 1995. The widespread use of this additive has been suggested to cause health risks, but limitations in data regarding its degradation products and their toxicity prevent an accurate evaluation. To monitor the organomanganese compounds, it is clearly advantageous to employ low-cost, high-sensitivity, manganese-specific instrumentation to perform speciation. In this work, instrumentation fitting these criteria was obtained by the combination of high-performance liquid chromatography (HPLC) with diode laser atomic absorption spectrometry (DLAAS) 例. Comparison of fluorimetric and inductively coupled plasma mass spectrometry detection systems for the determination of aluminium species in waters by high-performance liquid chromatography（Ben Fairman, et al., Analyst, April 1998, 123: 699） The comparison of element-specific detection using HPLC–ICP-MS with an established HPLC–fluorimetric method for aluminium speciation in waters is described. This comparison allowed the identification of some problems with a fluorimetric detection method based around 8-hydroxyquinoline-5-sulfonic acid, particularly its comparatively poor selectivity and Al-species dependent response factors. The power of ICP-MS as a detector for HPLC systems is demonstrated by the simultaneous detection of Al, Mg, Zn, and Fe. The increased selectivity of ICP-MS over molecu