内蒙古乌拉特中旗传统乳制品中乳酸菌的分离鉴定---.doc

本科毕业论文 内蒙古乌拉特中旗传统乳制品中乳酸菌的分离鉴定 学 院：食品科学与工程 专 业：食品科学与工程 学 号： 姓 名：张永亮 指导教师：孟和毕力格 职 称：教 授 论文提交日期：二Ο一五年六月 摘 要 传统乳制品是乳酸菌的天然栖息环境，为了认识和研究干旱半荒漠地区传统乳制品中乳酸菌菌群结构，并分离保藏该乳酸菌资源，以采自内蒙古乌拉特中旗的35份传统乳制品样品（19份酸牛奶，7份奶油，2份奶酪，7份鲜牛奶)为研究对象，运用纯培养技术和16S rRNA基因序列分析方法对样品中乳酸菌进行分离鉴定。结果显示：从35份样品中共分离获得属于12个种和亚种的124株乳酸菌，即Lac. lactis subsp. lactis（48株，占分离株38.71%）、L. plantarum（32株，占25.81%）、Leu. mesenteroides（22株，占17.74%）、L. paracasei（6株，占4.84%）、L. helveticus（5株，占4.03%）、L. rhamnosus（3株，占2.42%）和L. brevis、L. diolivorans各2株（分别占1.61%），且Leu. pseudomesenteroide、E. durans、E. sulfurous、L. buchneri各1株（分别占0.81%）。其中以Lac. lactis subsp. lactis和L. plantarum为样品中优势菌群，其次Leu. mesenteroides和L. paracasei的分离数量较多。 关 键 词：传统乳制品；乳酸菌；分离鉴定 Isolation and identification of Latic acid bacteria from traditional dairy products in Wulate Zhongqi Abstract Traditional dairy products were the natural habitats of lactic acid bacteria. In order to reveal and research the population structure of lactic acid bacteria, and preserve lactic acid bacteria from traditional dairy products in arid and desert areas. Lactic acid bacteria were isolated and identified using pure culture method and 16S rRNA gene sequencing from sixty nine samples of traditional dairy products, including yogurt, cream, cheese and milk, which were collected from pastoral of Wulate Zhongqi areas of Inner Mongolia.The results showed as follows: 124 isolates from 35 samples of Wulate Zhongqi of Inner Mongolia were identified into 12 species or subspecies, including Lac. lactis subsp. lactis (48 strains, account for 38.71% of the total isolate), L. plantarum (32 strains, account for 25.81%), Leu. mesenteroides (22 strains, account for 17.74%), L. paracasei (6 strains, account for 4.84%), L. helveticus (5 strains, account for 4.03%), L. rhamnosus (3 strains, account for 2.42%) and L. brevis, L. diolivorans (2 strains, account for 1.61% respectively) and Leu. pseudomesenteroide、E. durans、E. sulfurous、L. buchneri (1 strains, account for 0.81% respe