广东豚草居群的遗传分化.PDF

广东农业科学 2012 年第 3 期 135 广东豚草居群的遗传分化 黄久香袁 刘宪宽袁 庄雪影袁 翟翠花袁 黄川腾袁 徐瑞晶 渊华南农业大学林学院/华南植物研究室袁广东 广州 510642冤 摘 要 利用 ISSR 技术分析了广东豚草渊A mbros ia artemisiifolia L.冤居群的遗传多样性及遗传分化遥 应用 11 条引物从 6 个居 群 182 个样品中扩增出186 个位点袁多态性位点比率渊PPB%冤为 98.92%遥 Shannon 信息指数渊I冤为0.5238 袁Nei 爷s 基因多样性指数(H) 为0.3540袁反映了广东豚草居群具有较高的遗传多样性遥基因分化系数( GST=0.3263)和AMOVA 分析均显示入侵广东的豚草居群 具有很高的遗传分化遥研究结果表明袁粤北与粤东豚草居群的遗传距离较近袁最南部的花都居群渊HD冤与其他居群遗传距离相对较 远袁说明向南部扩散的豚草居群与粤北的居群已产生了较大的遗传分化遥 关键词 豚草曰 入侵植物曰 遗传多样性曰 ISSR 中图分类号 Q948.1 曰Q949.783.5 文献标识码 A 文章编号 1004-874X渊2012冤03-0135-04 Genetic divergence of populations of Ambrosia artemisiifolia L. in Guangdong HUANG J iu-xiang, LIU Xian-kuan, ZHUANG Xue-ying, ZHAI Cui-hua HUANG Chuan-teng, XU Rui-ji ng 渊South China Researc h Laboratory of Botany/College of Forestry, South China A gricultural Univ ersity, G uangzhou 510642, China冤 Abstract 院The population genetic diversity and differentiation of the invasive species, A mbrosia artemisiifolia, in Guangdong were analyzed using ISSR. A total of 11 primers were used and 186 bands were amplified from 182 samples of the six populations. The percentage of polymorphic loci was 98.92% . Shannon 爷s information index was 0.5288. Nei 爷s index was 0.3540. The data suggested genetic diversity of Guangdong populations was high. Both GST (0.3263) and A MOVA showed a high differentiation among populations. The populations in the northern and eastern parts of Guangdong had close genetic similarity, but the southernmost population of Hua