K252A对内毒素诱导急性肺损伤大鼠的保护作用研究.docVIP

K252a对内毒素诱导急性肺损伤大鼠的保护作用研究 毕业论文网 毕业论文 【摘要】nbsp; 目的 观察K252a（MLK3阻断剂）对内毒素性急性肺损伤大鼠肺病理组织学改变及生存率的影响。方法 采用尾静脉注射脂多糖（lipopolysaccharide，LPS）复制内毒素性急性肺损伤模型。90只大鼠随机分为6组（n=15）：对照组(control组)、LPS 组、K252a 50 μg/kg组（K50组）、K252a 75 μg/kg组（K75组）、K252a 100 μg/kg组（K100组）和溶剂对照组（DMSO组）。模型制备成功后6 h取肺脏进行光镜检查，观察48 h内大鼠死亡情况并比较累计生存率。结果 48 h内LPS组、K50组、K75组和K100组以及DMSO组大鼠生存率分别为6.3%、27%、72%、57%及6.7%。光镜下可见LPS组大鼠肺组织结构明显被破坏，主要表现为肺泡内出血、肺间质水肿、肺泡萎陷以及肺组织内大量炎性细胞浸润。与LPS组相比，预先应用K252a组肺组织的损伤程度较轻微、肺间隔轻度增宽、无明显出血、少量炎性细胞浸润，尤其K75组，减轻程度最明显。结论 K252a延迟LPS大鼠死亡时间，并呈现剂量依赖性，75 μg/kg K252a剂量可以明显提高其生存率。并可改善内毒素性急性肺损伤大鼠肺脏的病理组织学结果。 【关键词】nbsp; K252a;MLK3;内毒素;急性肺损伤；大鼠;生存率 Protective effects of K252a on endotoxin-induced acute lung injury in rats nbsp;nbsp;nbsp; endotoxin-induced acute lung injury in rats nbsp;nbsp;nbsp; ZHANG Maoyin1, CHEN Long1, CHENG Qin1, ZHANG Wenwen1, LIU Su2, WANG Guanglei2 , LIU Gongjian2 nbsp;nbsp;nbsp; (1.Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou, Jiangsu , China; nbsp;nbsp;nbsp; 2.Department of Anesthesiology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu ) nbsp;nbsp;nbsp; Abstract: Objectivenbsp; To investigate the effect s of K252a (MLK3 blocker) on the pathohistological changes in the lungs and the survival rate in rats with endotoxin-induced acute lung injury. Methodsnbsp; Rats models of acute lung injury were established by administration of lipopolysaccharide (LPS) as endotoxin in the caudal vein. 90 healthy male Sprague-Dawley rats were randomly divided into 6 groups (n=15): control group, LPS group, K252a 50 μg/kg group (K50), K252a 75 μg/kg group (K75), K252a 100 μg/kg group (K100) and vehicle control group (DMSO). Each group was sampled 6 h after administration of LPS for light microscopy examination. The deaths during 48 h in each group were observed and the accumulated survival rates were compared. Resultsnbsp; Within 48 hours, the survival rates of LPS group, K50 group, K75 group, K100 group and DMSO group were 6