[理学]基因组测序技术.ppt

ChIP Sequencing Alternative Splicing Wang et al. 2008 CNV Korbel et al, 2007 Metagenomics 谢谢 基因组测序技术 付永贵 Sanger Method Two Strategies Scaffolding Second-generation Sequencing Pyrosequencing Reversible terminator Sequencing by ligation Pyrosequencing Reversible Terminator Ju et al. 2006 Ligation Sample Preparation I. Roche 454 II.Solexa 5-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3 |||||||||||| 3-GTTCGTCTTCTGCCGTATGTCGAGAAGGCTAG-PO4-5 5-PO4-gatcggaagagctgtatgccgtcttctgcttg-3 |||||||||||| 3-tctagccttctcgcagcacatccctttctcaca-5 AATGATACGGCGACCACCGA GATCTACACTCTTTCCCTAC ACGACGCTCTTCCGATCT 3’TCTAGCCTTCTCGAGCATAC GGCAGAAGACGAAC5’ Complete Genomics Third generation sequencing Helicos BioSciences Pacific Biosciences Sample preparation and standard sequencing Strobe sequencing Application Genome Sequencing Ancient DNA sequencing CNV ChIP-sequencing and Epigenetics Gene Transcriptome Alternative Splicing Meta Genomics Genome Sequencing De novo sequencing Re-sequencing Target sequencing 1000 Genomes Project Target Re-sequencing Nimblegen Agilent Ancient DNA Briggs, 2009