大枣色泽与5-羟甲基糠醛含量相关性分析-中国中医药信息杂志.PDFVIP

2016 年8月第 23卷第8期 中国中医药信息杂志 ·83· ·中药研究与开发· 大枣色泽与 5-羟甲基糠醛含量相关性分析 吴翠，刘超，巢志茂 中国中医科学院中药研究所，北京 100700 摘要：目的 建立大枣中 5-羟甲基糠醛（5-HMF ）的高效液相色谱含量测定方法，对大枣色泽与5-HMF 含量的相关性进行探索。方法 收集多种色泽的市售大枣及其制品，采用高效液相色谱法测定 5-HMF 的含量。 结果 大枣制品中 5-HMF 的含量明显高于大枣中的含量，大枣中的 5-HMF 含量为 0.052 ～6.631 mg/100 g ，大 枣制品中为 3.324 ～39.71 mg/100 g。随着大枣的色泽逐渐加深，5-HMF 的含量显著升高。结论 本研究建立 了大枣及其制品中 5-HMF 的高效液相色谱测定方法。大枣中 5-HMF 的含量与色泽有关，建议优质大枣中 5-HMF 的限量指标为 1 mg/100 g。 关键词：大枣；5-羟甲基糠醛；色泽；麦拉德反应；高效液相色谱法 DOI：10.3969/j.issn.1005-5304.2016.08.022 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2016)08-0083-04 Analysis on the Correlation between Colors and Contents of 5-Hydroxymethylfurfural in Jujubae Fructus WU Cui, LIU Chao, CHAO Zhi-mao (Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China) Abstract: Objective To establish an HPLC method for content determination of 5-hydroxymethylfurfural (5-HMF) in Jujubae Fructus; To explore the correlation between colors and 5-HMF contents. Methods Some samples of Jujubae Fructus and the products with different colors were collected. The contents of 5-HMF were determined by HPLC. Results The contents of 5-HMF in Jujubae Fructus products are significantly higher than those in Jujubae Fructus.The contents of 5-HMF were among 0.052–6.631 mg/100 g in Jujubae Fructus and 3.324–39.71 mg/100 g in Jujubae Fructus products. As the color gradually deepen, the content of 5-HMF increases significantly. Conclusion An HPLC determination method for 5-HMF in Jujubae Fructus was established. The contents of 5-HMF in Jujubae Fructus and its products are associated with colors. It is recommended that the limit content of 5-HMF in Jujubae Fructu