透明质酸软骨链蛋白探针对肺肿瘤小鼠放疗靶区-南方医科大学学报.PDFVIP

2012;32(3) 南方医科大学学报（J South Med Univ ） ·301 · 临床研究 基础研究 透明质酸软骨链蛋白探针对肺肿瘤小鼠放疗靶区勾画的可行性 分析 梁智欣，强永刚，廖永华 广州医学院实验核医学教研室，广东 广州 510182 摘要：目的提取牛鼻HA 软骨链蛋白（HA-CLP），确定其对化学致癌小鼠模型肿瘤边界的指示情况，为肺肿瘤影像学感兴趣区 125 （ROI）边界的勾画提供实验依据。方法 建立化学致癌小鼠肺肿瘤模型，用亲和层析法从牛鼻软骨分离纯化HA-CLP ，后以 I 标记HA-CLP 行放射性自显影显像研究，并对肺肿瘤行免疫组织化学分析及蛋白免疫印迹分析。结果 实验收获HA-CLP （241.8±63.4）mg （n=5），抽提蛋白率为2.42% ；肺肿瘤放射性自显影在2 h 显像最清晰；免疫组织化学在2 h 能最清晰区分肿瘤边 界，其HA-CLP 免疫信号最强；肿瘤HA-CLP 蛋白免疫印迹均在2 h 条带显示最清晰。结论 自提物HA-CLP 具HABP 家族免疫 原性，通过应用自提物HA-CLP 放射自显影实验以及组织免疫组织化学提示HA-CLP 对肺肿瘤边界有良好的指示作用。 关键词：透明质酸软骨链蛋白；肿瘤模型；肿瘤边界；放疗靶区；肺肿瘤 中图分类号：R730.55 文献标志码：A 文章编号：1673-4254（2012）03-0301-05 DOI: CNKI:44-1627/R1604.014 /kcms/detail/44.1627.R1604.014.html Feasibility of cartilage link protein of hyaluronic acid for defining radiotherapeutic target volume in a mouse model of lung tumor LIANG Zhixin, QIANG Yonggang, LIAO Yonghua Department of Experimental Nuclear Medicine, Guangzhou Medical College, Guangzhou 510182, China Abstract: Objective To investigate the feasibility of using cartilage link protein of hyaluronic acid (HA-CLP) for defining the tumor boundary in a mouse model of lung carcinoma. Methods Lung carcinoma was induced in KM mice by chemical carcinogenesis. HA-CLP separated from bovine cartilage and purified by affinity chromatography was labeled with 125I for autoradiography. Immunohistochemical analysis and Western blotting were used to examine the efficiency of HA-CLP in defining the boundaries of the lung tumors. Results With autoradiography, the clearest image of lung cancer was obtained at 2 h. With immunohistochemical method, the tumor boundary was the most clearly displayed at 2 h wh