两株绿脓杆菌对石油污染土壤的修复作用-微生物学报.PDFVIP

生 物 工 程 学 报 黄磊 等/两株绿脓杆菌对石油污染土壤的修复作用 957 Chinese Journal of Biotechnology /cjbcn June 25, 2017, 33(6): 957−967 DOI: 10.13345/j.cjb.160472 ©2017 Chin J Biotech, All rights reserved ·环境生物技术· 黄磊，赵婷婷，贺赟，刘一琳，刘畅，靳大耀，贾晓强 300072 赟 , , , . . , 2017, 33(6): 957–967. Huang L, Zhao TT, He Y, et al. Bioremediation of oil-contaminated field by two Pseudomonas aeruginosa strains. Chin J Biotech, 2017, 33(6): 957–967. 摘 要: 本文旨在研究环境条件下微生物对石油污染土壤的修复情况。从矿井周边土样定向筛选出两株绿脓 杆菌，摇瓶降解实验发现，两菌混合培养 10 d 原油降解率达到95.67% ，比单菌培养提高至少32% ，即两菌对 原油降解具有协同作用。根据降解实验结果制备了混合修复菌剂，并且人工构建石油污染场地，展开中试场地 修复试验，模拟不同的操作条件下土壤中原油的降解情况。经 60 d 修复发现，添加了菌剂的场地，石油烃含 量下降趋势明显，每克土壤中石油烃含量从初始的 0.8%降至 0.1%–0.3% ，其中额外添加有机肥作为补充碳氮 源的场地，总石油烃降解率最高，达到85.28%。而未添加菌剂的对照组石油烃含量仅减少25.85% 。 : 石油污染，生物修复，混合菌剂，协同作用，场地试验 Bioremediation of oil-contaminated field by two Pseudomonas aeruginosa strains Lei Huang, Tingting Zhao, Yun He, Yilin Liu, Chang Liu, Dayao Jin, and Xiaoqiang Jia Key Laboratory of Systems Bioengineering (Ministry of Education), Department of Biological Engineering School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China Abstract: We studied the remediation of petroleum-contaminated soils. Two Pseudomonas aeruginosa strains were Received: December 8, 2016; Accepted : March 20, 2017 Supported by: National Basic Research Program of China (973 Program) (No. 2014CB745100), National Natural Science Foundation of China (No. , Tianjin Research Program of Application Foundation and Advanced Technology (No. 14JCQNJC06700), Tianjin Penglai 19-3 Oil Spill Accident Compensation P