高效液相色谱法同时检测6种甜味剂.pdfVIP

※分析检测 食品科学 2011, Vol. 32, No. 06 165 蒋晓彤，陈国松，姜玲玲，赵艳丽 (鞍山市产品质量监督检验所，辽宁 鞍山 114006) 建立同时测定 6 种人工合成甜味剂阿斯巴甜、糖精钠、甜蜜素、安塞蜜、纽甜、甜聚糖甙的高效液相 色谱分析方法。以 Platicil ODS 柱为分离柱，20mmol/L 硫酸铵缓冲溶液(pH4.4)- 乙腈为流动相，进行梯度洗脱。 采用二极管阵列检测器进行检测，整个分离过程在 30min 内完成。6 种甜味剂在 0.4～120mg/L 范围内其质量浓度与 峰面积的线性关系良好，相关系数为0.99967～0.99998，在4.0～10.0mg/kg 范围内，样品加标回收率为85%～107%； 相对标准偏差小于 3.2% 。该方法简便、快速，净化效果较好，可用于食品中6 种甜味剂的同时测定。 高效液相色谱( H P L C ) ；人工合成甜味剂；糖精钠；甜蜜素；安塞蜜；阿斯巴甜；甜聚糖甙；纽甜 Simultaneous HPLC Determination of 6 Sweeteners JIANG Xiao-tong ，CHEN Guo-song ，JIANG Ling-ling ，ZHAO Yan-li (Anshan Institute of Products Quality Supervision and Inspection, Anshan 114006, China) Abstract ： A reversed-phase high performance liquid chromatography (RP-HPLC) method was established to simultaneously determine six artificial sweeteners including aspartame (ASP), sodium saccharin (SA), sodium cyclamate (SC), acesulfame potassium (AK), neotame (NTM) and stevioside. The separation was carried out on a Platicil ODS (250 mm × 4.6 mm, 5μm) column through gradient elution using 20 mmol/L (NH4)2 SO4-acetonitrile (pH 4.4) as mobile phase. The whole separation process was completed in 30 min before diode array detection. An excellent linear relationship between peak area and sweetener concentration in the range of 0.4 －120 mg/L with a correlation coefficient varying from 0.99967 to 0.99998 was observed. The recovery rates of spiked samples in the range of 4.0 －10.0 mg/kg ranged from 85% to 107% with a relative standard deviation less than 3.2%. Therefore, this method is characteristics of simple operation, rapid detection and excellent purification efficiency so that it can be used for the routine analysis of these sweet additives in foods and bevera