基于TSP与直流电法的浅埋隧道超前预报.PDFVIP

HEREDITAS (Beijing) 2008 11 , 30(11): 1411―1416 ISSN 0253-9714112 研究报告 DOI: 10.3724/SP.J.1005.2008.01411 HLA 1, 2 1 1 郝向稳 , 邓立彬 , 曾长青 1. , 101300; 2. , 100049 : 人类白细胞抗原(Human leukocyte antigens, HLA)是人类基因组中已知多态性最高的基因家族, 为人类面 对异源多变的外界生物分子所必须。以往对HLA 多态性形成的研究多集中在互换式重组机制上, 文章研究了基 因转换这一重要的多态性形成机制在HLA-DRB 基因多态性形成中的作用。应用已知的各基因座的等位基因序 列对其进行多态性分析表明, HLA-DRB 是一高度多态的基因家族。用Ester Betran 模型检测到32 个基因转换的 区域, 最小的基因转换区域长2 bp, 最远差异位点间隔为204 bp 。在71~75 、18~221 等几个区域上出现基因转 换的频数高, 成为基因转换的热点。进一步的分析显示, 71~75 、205~217 等热点区域分别与东方人、高加索人 这两个人群密切相关, 提示基因转换的热点可能具有一定的人群特异性。 : HLA ; 多态性; 基因转换; 热点 The contribution of gene conversion at the same chromosome to the HLA diversity 1, 2 1 1 HAO Xiang-Wen , DENG Li-Bin , ZENG Chang-Qing 1. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China; 2. Graduate School of Chinese Academy of Sciences, Beijing 100049, China Abstract: HLA is the most polymorphic gene family in human genome, which is imperative for human to face numerous heterogeneous bio-molecules. Previous studies on the formation of HLA polymorphism have been focused on gene cross- over. Here, we investigated the contribution of gene conversion, which is an important mechanism to generate polymor- phism at shaping different patterns of HLA-DRB genes. Analysis of all known HLA-DRB haplotypes and alleles demon- strated that this was a highly polymorphic gene family. Using Ester Betran’s algorithm, 32 gene conversion regions were identified. The minimal conversion tract was as short as 2 bp, and the maximum interval between two furthest SNPs was 204 bp. Moreover, gene conversion occurred more frequently in c