Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a精选.pdf

Journal of Biotechnology 148 (2010) 204–207 Contents lists available at ScienceDirect Journal of Biotechnology j o u rn al h omepage: w w w. elsev /locate/jbiotec Short communication Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential of artificial bio-synthetic pathway using thermophilic enzymes Kohsuke Honda a,∗ , Shohei Maya a , Takeshi Omasa a , Ryuichi Hirota b , Akio Kuroda b , Hisao Ohtake a a Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan b Department of Molecular Biotechnology, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan a r t i c l e i n f o a b s t r a c t Article history: Six thermophilic enzymes from Thermus thermophilus were used to construct an ‘artificial bio-synthetic Received 6 April 2010 pathway’ for the production of 2-deoxyribose 5-phosphate from fructose. By a simple operation using Received in revised form 25 May 2010 six recombinant Escherichia coli strains producing the thermophilic enzymes, respectively, fructose was Accepted 7 June 2010 converted to 2-deoxyribose 5-phosphate with a molar yield of 55%. © 2010 Elsevier B.V. All rights reserved. Keywords: Thermophilic enzyme Artificial bio-synthetic pathway 2-Deoxyribose 5-phosphate 2-Deoxyriboaldolase Thermophilic enzymes are getting increased use in bio- synthesis of 2-deoxyr