丙型肝炎病毒教材教学课件.ppt

*;HAV;;0.05;1;病毒性肝炎发病情况;急性丙肝;中国HCV感染的诊断和治疗现况;High titer NANBH chimpanzee plasma ;;Southern Blots with Clone 81;Northern Blots with Clone 81;Western blot;HCV was the first virus to be isolated and characterized solely by molecular methods, ie without culture methods;HCV病原学;;;;;;;;Genomic RNA is infectious ;;;;HCV receptors/entry factors;;-HCV core protein drives assembly at the lipid droplet （LD） -LD is bound by core, then NS5A and other NS proteins;;;DAA的临床研发;HCV proteins interact with many host-cell factors and impact on a wide range of cellular activities;HCV Life Cycle: Key Features;HCV Life Cycle: Key Features;丙型肝炎流行病学 Worldwide Prevalence of HCV infection ;HCV Infection Prevalence in China;HCV Infection Prevalence in China;Evolution of anti-HCV antibody assays;;Hemodialysis, 10 cases, Wulate;HCV Infection Prevalence in China;HCV 变异;HCV分为6个基因型 中国以1b和2a基因型常见，1b型对IFN应答差 基因6型主要见于香港和澳门;HCV Genotypes and Quasispecies;Quasispecies are kind of like siblings: highly related yet genetically distinct;小结;核心抗原检测在HCV/HIV共感染人群的应用 ;HCV is common in HIV patients (approx 25-40% in China and worldwide);HCV is a more serious disease in coinfected patients than in mono-infected;HIV accelerates HCV liver disease especially when CD4 declines;HIV 感染慢性丙肝的筛查与诊断;实验设计; A：354份样本分为5组： HIV- CHC (129, 36.44%) HIV+ CHC (98, 27.68%) HIV- SVC (65, 18.36%) HIV+ SVC (44, 12.43%) HIV单感染组 (18, 5.09%);(A) ;HCV病毒载量与HCV核心抗原的比值在HCV单感染人群中要显著高于HCV/HIV混合感染人群（p=0.0414) 是否提示HCV核心抗原水平更直接反映了宿主的免疫状态？;HIV- CHC: b(r=0.8083, p0.001）；HIV+ CHC（r=0.9524, p0.001）;中国流行HCV亚型HCV-1b和HCV-2a在HCV核心抗原浓度和HCV RNA载量之间的相关性没有显著差异。;Result-3 HCV 抗体水平、核心抗原浓度与CD4计数;Conclusion;*