高效液相色谱法测定竹茹提取物及其不同部位中的腺苷含量龚金炎.docVIP

高效液相色谱法测定竹茹提取物及其不同部位的腺苷含量 龚金炎1,2,3,4(，葛青1,2，张蕾1,2，陈丽春1,2，毛建卫1,2，黄俊1,2刘士旺1,2，1,2，张英3 （1.浙江科技学院生物与化学工程学院，浙江杭州，310023; 2.浙江省农产品化学与生物加工技术重点实验室，浙江 杭州310023; 3.浙江大学生物系统工程与食品科学学院，浙江 杭州 310029; 4. 绍兴市质量技术监督检测院，浙江 绍兴，312000） 摘 要：建立一种反相高效液相色谱（RP-HPLC）法测定竹茹提取物及其不同部位的腺苷含量的方法。采用RP-HPLC法；Luna C18 ODS柱（4.6×250mm，5 μm），乙腈-0.01 mol/L磷酸缓冲溶液（pH=6.5）；体积流量：1.0 mL/min；进样量：10 μL；柱温：40 ℃；检测波长：257 nm。结果 采用RP-HPLC法竹茹提取物及其不同部位的腺苷含量，在0.121–124 μg/mL范围内线性关系良好（R2= 0.9999），平均回收率为98.3%，RSD为3.11%。本方法简便、灵敏、准确，可普遍应用于竹茹提取物的质量研究和品质控制。 关键词：竹茹提取物；腺苷；高效液相色谱 Determination of Adenosine in Extract of Bamboo Shavings by HPLC G Jin-yan 1,2,3,4, ZHANG Lei 1,2, CHEN Li-chun 1,2, GE Qing 1,2, MAO Jian-wei 1,2, HUANG Jun 1,2, LIU Shi-wang 1,2, WANG Yong-jiang 1,2, ZHANG Ying 3 (1.School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310029, China;2.Zhejiang provincial Key Lab for Chem Bio Processing Technology of Farm produces; Zhejiang University of Science and Technology, Hangzhou 310029, Zhejiang, China; 3. College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, Zhejiang, China; 4 Shaoxing Testing Institute of Quality Technical Supervision, Shaoxing 312000, Zhejiang, China) Abstract: To develop a method for determination of adenosine in extract of bamboo shavings by RP-HPLC. RP-HPLC method was used with Luna C18 ODS (4.6×250mm, 5 μm) column. The mobile phase was acetonitrile -0.01 mol/L phosphate buffer solution（pH=6.5） with 1.0 mL/min flow rate; The column temperature was 40℃; The detection wavelength was set at 257 nm. RP-HPLC method was used for the determination adenosine; The calibration curve of adenosine was linear under the content 0.12–124 μg/mL; The linearity the average recovery was from 98.3%, RSD was 3.11%. Conclusion The method is simple, sensitive and accurate. The method can be adapted to control the quality of extract of bamboo shavings. Key words: Bamboo shavings; Adenosine; HPLC 竹茹（Bamboo shavings，Caulis Bamf