A thermosensitive chitosan-based hydrogel barrier for post-operative adhesions’ prevention.pdf

Biomaterials 30 (2009) 5534–5540 Contents lists available at ScienceDirect Biomaterials journal homepage: www.elsevi /locate/biomaterials A thermosensitive chitosan-based hydrogel barrier for post-operative adhesions’ prevention Chang-Zheng Wei a, Chun-Lin Hou a, *, Qi-Sheng Gu b, Li-Xia Jiang b, Bin Zhu b, Ai-Lian Sheng b a Department of Orthopedics in Changzheng Hospital of Second Military Medical University, Shanghai 200003, China b Qisheng Biologic Agent Limited Company in Shanghai, Shanghai 201106, China a r t i c l e i n f o a b s t r a c t Article history: Post-operative peritoneal adhesions are common and serious complications for surgeons. They can Received 5 March 2009 cause pelvic pain, infertility, and potentially lethal bowel obstruction. We synthesized injectable Accepted 19 May 2009 hydrogels that formed by chemical modification through grafted hydrobutyl groups to chitosan chains. Available online 3 August 2009 Gelation of hydroxybutyl chitosan (HBC) occurs in less than 60 s. Once formed, it can also be recovered completely. The residue time of hydrogels can extend to 4 weeks in Kunming mice. HBC hydrogels Keywords: showed mild cytotoxicity to mice fibroblast cell (L929) and human vascular endothelial cell (ECV-304) Post-operative adhesions in vitro and were biocompatible in the murine muscles, causing no adhesi