硕士论文-鹿蹄草素对金黄色葡萄球菌抑制作用及其机理的研究精选.docVIP

分类号 学校代码 I C S 学 号 鹿蹄草素Study on Bacteriostatic Action and Antimicrobial Mechanism of Pyrolin against Staphylococcus aureus 申 请 人： 学科门类： 工学 学科专业： 食品科学 研究方向： 食品微生物的研究与利用 指导教师： 论文提交日期：二○○七年五月 摘 要 本课题通过采用常规抑菌实验、电导率测定、扫描电镜观察及RT-PCR等实验方法，从菌体生长状态、生长曲线、细胞膜通透性、细胞外观形态、菌内转肽酶femB 基因的RNA转录水平等方面，对鹿衔草中主要抗菌活性成分鹿蹄草素对金黄色葡萄球菌抑制作用及其机理进行研究，结果表明:鹿蹄草素能有效抑制金黄色葡萄球菌的生长，其最小抑菌浓度MIC为0.16mg/ml鹿蹄草素对金黄色葡萄球菌还具有较强的杀菌作用，最小杀菌浓度为0.2mg/ml鹿蹄草素作用于金黄色葡萄球菌后，膜通透性与空白对照组比较显著提高；扫描电镜观察，femB基因的mRNA水平；作用8h时，femB基因的mRNA水平已仅为的1/femB 基因转录为mRNA过程受到抑制，femB酶的翻译表达量降低，干扰肽聚糖的合成，Study on Bacteriostatic Action and Antimicrobial Mechanism of Pyrolin against Staphylococcus aureus Abstract For studying on bacteriostatic action and antimicrobial mechanism of Pyrolin against Staphylococcus aureus, by using the common experimental way in microbiology, Mensuration of conductivity meter, scanning electron microscope, RT-PCR and so on, Staphylococcus aureus were studied in the growth curves, the membrance patency, the structure under scanning electron microscope and mRNA level of femB gene and so on, who is normal and effected by different Pyrolin who is main actival composition in Pyrolaceae. The results show that Pyrolin could inhibit the growth of Staphylococcus aureus, and the minimum inhibitory concentration was 0.16mg/ml. Pyrolin had strong bactericidal action against Staphylococcus aureus, and the minimum bactericidal concentration was 0.2mg/ml. The membrance patency of the cells affected by Pyrolin sharply increased compared with that of the normal cells. Observation under scanning electron microscope revealed that structure of the cells of Staphylococcus aureus had been significantly destroyed by Pyrolin, and the cells wall were melted. The cells were stick by the melt together, and the bounds among cells got blur. They indicated that the antimicrobial activity and the bactericidal function of Pyrolin against Staphylococcus aureus w