Expression of adipogenesis derived genes during differentiation of 脂肪细胞分化过程中的基因表达的来源.docVIP

EXPRESSION PROFILES OF ADIPOGENESIS DERIVED GENES DURING DIFFERENTIATION OF ABDOMINAL BOVINE PREADIPOCYTES Mohamed Mohamed Soliman1*, Zein Shaban Ibrahim2,3, Khlood Elbohy4 Departments of Biochemistry1 , Physiology2,and Toxicology,4, Faculty of Veterinary Medicine, Benha1, Kafr El-Sheikh2 and Zagazig universities, Egypt. 3Laboratory of Toxicology, Graduate School of Veterinary Medicine, Hokkaido University, Japan. *Correspondence to: Mohamed Mohamed Soliman , Department of Physiology and Biochemistry, Faculty of Veterinary Medicine, Kafr El-Sheikh, Egypt Email address: ab_ah_sh_mohamed@ ABSTRACT Fat cells in the body present in immature stage (preadipocyte). Under certain conditions such as obesity, nutrient with high calorie intake availability, these cells changed into mature cells by triglycerides accumulation in its cytoplasm and ability to express certain genes in a process named adipogenesis derived genes. Such gene expression was highly investigated in humans and rodents but less data was recorded in cattle although fattening is important in veterinary field for meat marbling. Bovine abdominal adipose tissue was isolated and the preadipocytes were induced for differentiation using insulin, dexamethasone and cholesterol for 12 days to test different gene expressions using RT-PCR analysis. The expression of adipocytes fatty acid binding protein (aP2), acylation stimulating protein (ASP), insulin regulated-glucose transporter-4 (GLUT-4) and peroxisome proliferator-activated receptor-? (PPAR-?) were increased in time dependent manner together with the degree of lipid accumulation in cells. Moreover, leptin expression did not show any alteration in its expression using this protocol for differentiation unlike adiponectin which shows an increase in the expression on day 10 and 12. These findings showed for the first time the expression of ASP, GLUT-4 and adiponectin during bovine adipocytes differentiation and their relation to fattening and peripheral glucose ut