转基因细胞株BaFmb1RAE对IKDC功能分子表达的影响.DOCVIP

转基因细胞株BaF3-mb15-RAE对IKDC功能分子表达的影响 钱莉，陆家辉，，，，龚卫娟，，季明春扬州大学医学院 病原生物学与免疫学教研室，江苏 扬州 225001 [基金项目] 国家自然科学基金（Noo.No.；江苏省自然科学基金（No. BK2010315）新世纪人才工程资助Project supported by the National Natural Science Foundation of China (NoNo.No. ，the Natural Science Foundation of Jiangsu Province (No.BK2010315)，the Science and Technology Innovation fund for undergraduates of the Educational Committee of Jiangsu Province, and the new century talents project of Yangzhou University. [作者简介] 钱莉（1979-），女，江苏扬州人，博士，，主要肿瘤免疫及免疫调节。[通作者]（，corresponding author），E-mail [摘要] 目的：探讨共同视黄酸早期转录因ε（retinoic acid early transcriptε，R1ε）和膜型IL-15对小鼠（IFN producing killer IKDC）的影响。方法：膜型IL-15BaF3-mb15细胞、表达RAE1ε的BaF3-RAE细胞和同时表达膜型IL-15和RAE1εBaF3-mb15-RAE细胞。通过体外细胞因子诱导产生骨髓来源的小鼠成熟DC，将丝裂霉素灭活后后流式细胞检测表面结果：BaF3-mb15细胞和BaF3-RAE细胞相比，BaF3-mb15-RAE细胞FasL（P﹤0.05）的表达上调，和的没有。结论：RAE-1ε和膜型IL-15协同作用促进。[关键词] 膜型IL-15；视黄酸早期转录因ε；[中图分类号] R730. [文献标识码] A RAE on the expression of functional molecules on IKDC Qian Li1, Lu Jiahui1, Qin Hongchao1, Rui Chenglei1, Chen Weiyan1, Jia Xiaoqin1, Fu Yi1, Gong Weijuan1, Tian Fang1, Hu Maozhi2, Ji Mingchun1 (1. Department of Etiology and Immunology, Medical School of Yangzhou University, Yangzhou 225001, Jiangsu, China; 2. Testing Center of Yangzhou University, Yangzhou 225001, Jiangsu, China) [Abstract] Objective: To study the effects of membrane-bound form of IL-15 in combination with retinoic acid early transcript 1ε (RAE1ε) on the expression of functional molecules on IFN producing killer DCs ( IKDCs ). Methods: Three derivatives of mouse pro-B lymphocyte cell line BaF3, expressing membrane-bound IL-15(termed BaF3-mb15), or RAE1ε (termed BaF3-RAE), or both membrane-bound IL-15 and RAE1ε (termed BaF3-mb15-RAE) were respectively constructed in the previous study. Mouse bone marrow mononuclear cells were induced into mature DCs by GM-CSF and IL-4 in vitro. Mature DCs were stimulated with mitomycin inactivated-BaF3 derivatives. After 3 days