GCMS测牙膏中二甘醇.doc

Gas Chromatography - Mass Spectrometry (GC-MS) Screening Procedure for the Presence of Diethylene Glycol and Ethylene Glycol in Toothpaste (Version 2.0) This method for Diethylene Glycol and Ethylene Glycol in toothpaste should be regarded as Level 1 - Urgent Use. The user should verify the performance of the method in their laboratory for each different matrix and pay particular attention to the recommended quality control elements. Users should document with their results the version or date of the method used. PURPOSE: This procedure was developed at the Forensic Chemistry Center (FCC) to examine toothpaste for the presence of Diethylene Glycol (and a related substance of similar toxicity, Ethylene Glycol) at levels of 1 mg/g (0.1% by weight) and above. SCOPE: This procedure has been shown to be capable of detecting both analytes at a level of 0.5 mg/g a variety of different brands of toothpaste. RESPONSIBILITY: It is the responsibility of the analyst to note any modifications to or deviations from this procedure in the documentation associated with the analysis. DEFINITIONS AND ACRONYMS: SAFETY CONSIDERATIONS: Appropriate safety measures should be employed when working with chemicals, pressurized gases and the instrumentation which is employed. Generally, these will be defined in each laboratorys safety plan. No extraordinary precautions are necessary here. EQUIPMENT AND SUPPLIES: Agilent 5975i GC-MS system equipped with a 30 m Stabilwax (crossbond Carbowax) capillary column (or equivalent) or other mass spectrometer system. Laboratory centrifuge capable of applying 5000 g to 15 mL centrifuge tubes. 15-mL polypropylene centrifuge tubes with screw caps. REAGENTS AND STANDARDS: Extraction Solvent: Water followed by the addition of Acetonitrile 1,3-Propanediol For use as an internal standard. Prepare a stock solution at 5.0 mg/mL in 50% aqueous acetonitrile. Add 0.050 mL of this solution to 0.500 mL of each sample extract in the autosampler vial prior to an