胚胎干细胞来源神经干细胞调控巨噬细胞的研究.pdfVIP

祝摇 文等摇 胚胎干细胞来源神经干细胞调控巨噬细胞的实验研究摇 第12期 ·1741· doi:10.3969/ j.issn.1000鄄484X.2016.12.003 胚胎干细胞来源神经干细胞调控巨噬细胞的实验研究淤 祝摇 文摇 袁亚迎摇 焦峰军摇 (咸阳市第一人民医院重症医学科,咸阳712001) 摇 摇 中图分类号摇 R392郾4摇 摇 文献标志码摇 A摇 摇 文章编号摇 1000鄄484X(2016)12鄄1741鄄04 [摘摇 要]摇 目的:观察胚胎干细胞(Embryonic stem cell,ESC)来源的神经干细胞(Neural stem cells,NSCs)对骨髓巨噬细 胞的增殖、吞噬及分泌细胞因子的影响,为探讨NSCs免疫调节及在重大疾病中的应用做铺垫。 方法:培养骨髓巨噬细胞,收 集胚胎干细胞来源NSCs(ESC鄄NSCs)的上清液处理巨噬细胞,然后利用磺酰罗丹明B(Sulforhodamine B,SRB)法检测增殖情 况;利用红荧光beads检测吞噬能力;ELISA法检各组TNF鄄琢和IL鄄1茁表达情况。 结果:成功从ESC获得具有NSCs特征的细 胞。 对照组、NSC组巨噬细胞增殖率分别为 100%、(126郾29依5郾41)%,beads 吞噬率分别为(70郾23依2郾57)%、(90郾32依 8郾49)%。 相比对照组,NSC组巨噬细胞TNF鄄琢和IL鄄1茁含量下降(P0郾05)。 结论:ESC来源的NSCs具有促进骨髓巨噬细胞 增殖及增强其吞噬能力,并抑制其炎性因子分泌,为NSCs免疫调节作用提供新的依据。 [关键词]摇 神经干细胞;胚胎干细胞;巨噬细胞;吞噬;细胞因子 Effect of embryonic stem cells鄄derived neural stem cells on macrophage in vitro ZHU Wen,YUAN Ya鄄Ying,JIAO Feng鄄Jun郾Department of Critical Care Medicine,the First People忆s Hospital of Xianyang City,Xianyang 712001,China [Abstract]摇 Objective:To explore the influence of embryonic stem cells鄄derived neural stem cells on the proliferation and secretion cytokines ofbonemarrow鄄derivedmacrophages郾Methods:Mousebonemarrowderivedmacrophageswereisolatedandcultured in L929 medium郾After macrophageswere treatedwith NSCs supernatant for3 days,SRB methodwasusedto detect the proliferation of macrophages郾The phagocytosis of macrophagesweredetectedby incubatingwith RFP鄄Beadsfor 1h郾Meanwhile,theexpressionofTNF鄄 琢 and IL鄄1茁 were detected by ELISA郾Results:NSCs were successfully induced from ESC郾In control group and NSC group,the proliferation rate of macrophageswere 100 % and (126郾29 依 5郾41)%,the phagocytosisratewere (70郾23 依 2郾57)% and (90郾32 依 8郾49)%郾Comparedtothecontrolgroup,thelevelsofIL鄄6,IL鄄1茁inmacrophagetreatedwithNSCsdecreased (P0郾05)郾Conclusion: ESC鄄derived NSCs can promote the proliferation and phagocytosis of bone marrow鄄derived macrophage,and suppress the secretion of pro鄄infla