胍基丁胺对星形胶质细胞缺血再灌注损伤的保护作用.DOCVIP

缺血再灌注损伤后星形胶质细胞iNOS的表达及胍丁胺的干预作用研究 杨美子1，张树平1，金勇君2* （1.滨州医学院 药理学教研室，烟台 264003； 2.滨州医学院附属医院 内分泌科，滨州 256603） 摘要：目的 观察星形胶质细胞缺血再灌注损伤后iNOS的表达及胍丁胺的干预作用。 方法 体外缺血再灌注法诱导损伤原代培养的星形胶质细胞，检测培养液中乳酸脱氢酶（LDH）NO生成量和诱导型一氧化氮合酶（iNOS）LDH漏出量。经流式细胞仪分析结果显示胍丁胺主要是降低细胞坏死率而对凋亡率则无显著性影响。与阳性对照组相比，对星形胶质细胞胍丁胺显著降低缺血再灌注损伤引起的NO产量，同时也减少了iNOS表达。 结论 胍丁胺对缺血再灌注损伤引起的星形胶质细胞具有保护作用，其可能的作用机制是通过降低iNOS、抑制炎症因子NO合成，从而降低星形胶质细胞的炎症坏死。 关键词：胍丁胺；缺血再灌注损伤；星形胶质细胞；诱导型一氧化氮合酶 The effect of agmatine on iNOS expression after ischemia - reperfusion injury in astrocyte Yang Mei-Zi1, Zhang Shu-Ping1, Jin Yong-Jun2* (1.Department of Pharmacology, Binzhou Medical University, Yantai 264003; 2.Department of Endocrinology, Affiliated Hospital of Binzhou Medical University, Binzhou 256603 ) Abstract: Objective To investigate the protective effect and related mechanisms of agmatine on murine brain astrocyte after ischemia-reperfusion injury. Methods The astrocytes were subjected to like ischemia-reperfusion injury (oxygen glucose deprivation-reperfusion, OGD-R). The efflux of lactate dehydrogenase (LDH) and flow cytometric analysis were determined. Also, the production of NO were measured, the expression of inducible NOS (iNOS) were detected by western blot analysis. Results The viability of astrocyte was increased and the efflux of LDH were reduced by agmatine treatmented. By FITC-Annexin V/PI staining, the data demonstrated that agmatine decreased the necrosis rate but the apotosis rate. And the contents of NO production were reduced, also the expression of iNOS were decreased by agmatine. Conclusion Agmatine has a protective effect on astrocyte after ischemia-reperfusion injury, which maybe related to its inhibitory effect on iNOS-NO pathway. Key words: agmatine; ischemia-reperfusion injury; astrocyte; iNOS 脑缺血再灌注能够诱导炎症反应和细胞凋亡，导致广泛的神经元损伤。如能减少星形胶质细胞等神经元周边细胞分泌炎症或凋亡相关因子，将会减少对神经元的损伤，促进修复和保护作用。星形胶质细胞作为神经元重要的支持细胞，在动物脑缺血实验中显示出功能异常活跃,可分泌多种营养因子、细胞因子等从而对受损脑神经元起到更好的保护和修复作用;同时星形胶质细胞也可以释放诱导和促进神经元损伤的凋亡或炎症相关因子来加速神经元损伤[1-2]。 在缺血再灌注损伤时NO既有神经毒性作用，又有神经保护作用，