以简约化标记辅助选择改良三个重要杂交水稻亲本的稻.docVIP

研究报告 Research Report 分子标记辅助选育聚合抗稻瘟病基因和抗白叶枯病基因的水稻改良新恢复系 田大刚1,2　陈在杰2　陈子强2　林艳2　周元昌1*　陈松彪2　王锋1,2* 1福建农林大学, 作物遗传育种与综合利用教育部重点实验室, 福州, 350002; 2福建省农业科学院生物技术研究所, 福建省农业遗传工程重点实验室, 福州, 350003 通讯作者，zwy_2002@163.com wf@ 摘　要　研究选择三个新育成杂交水稻恢复系闽恢3189、闽恢3229和闽恢6118为受体亲本，以携带抗稻瘟病主基因Pi9的C750和抗白叶枯病主基因Xa23的C682为供体亲本，利用分子标记辅助选择和田间鉴定选择相结合方法，经过多代回交、自交，获得个导入Pi9或Xa23或Pi9+Xa23的水稻恢复系分子改良系。以19个福建近年稻瘟菌优势菌株和一个白叶枯病强致病菌株P6进行人工接种，结果表明，Pi9或Xa23的导入明显提高了水稻恢复系分子改良系的稻瘟病或白叶枯病抗性。分子改良系及其杂交组合的有效穗数、千粒重和单株产量等与受体亲本及其杂交组合的相关农艺性状无明显差异。这些抗性分子改良系有望升级原有受体亲本，在生产中具有良好应用前景。 关键词：水稻恢复系, 稻瘟病, 白叶枯病, 分子标记辅助选择, Pi9/Xa23 Developing Improved Lines of Three Rice Restorers Pyramided Resistant Genes to Blast and Bacterial Blight by Marker-assisted Selection Tian Dagang 1,2　Chen Zajie 2　Chen Ziqiang 2　Lin Yan 2　Zhou Yuanchang 1*　Chen Songbiao 2　Wang Feng 1,2* 1 Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agricultural Forestry University, Fuzhou, 350002; 2 Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fujian Key Laboratory of Genetic Engineering for Agriculture, Fuzhou, 350003 *Corresponding authors: zwy_2002@163.com; wf@ Abstract　A broad-spectrum blast resistance gene Pi9 from a donor line C750, and a bacterial blight (BB) resistance gene Xa23 from a donor line C682 were introgressed into three novel Indica rice restorer lines Minhui 3229, Minhui 3189, and Minhui 6118 by marker-assisted backcrossing in combination with stringent phenotypic selections. A total of eight genetic improved lines carrying Pi9, Xa23 or Pi9+Xa23 were developed. Inoculations of the developed lines carrying Pi9 with 19 representative blast isolates collected from Fujian province, and of the developed lines carrying Xa23 with a destructive Xanthomonas oryzae pv. oryzae strain PXO99 (P6) revealed that the improved rice restorers conferred significantly enhanced resistance to blast, or to BB. Investigation of major agronomic characteristics indicated that the improved line