应用FACS检测CIK的抗肿瘤活性.pdf

·l· ·论 著· 应用FACS检测CIK的抗肿瘤活性木 蒋东霞，赵婷茹1，杨晓博，胡杰英，徐虹，宋永平 河南省肿瘤研究所郑州市450008 摘要 目的探讨体外CIK细胞(cytokine—inducedkiller)对肿瘤细胞的细胞毒作用。方法从健康人 CFSE标记靶细胞以区分效应细胞，再以PI染色，FACS检测靶细胞的死亡率。结果CIK细胞高度表达CD3+、 感HI．ri0、Hela均表现出较强杀伤活性。结论细胞因子IFN 1、CD3McAb、IL一2体外诱导的单核细胞具有强大 的增殖力和杀伤活性及非MHC限制性。 关键词 CIK细胞；细胞毒性；流式细胞术 中图分类：号R730．45文献标识码：A DetectionofCIKAnti——Tumorvia ofFACS ActivityApplication al JIANG Xiaobo，et Dongxia，ZHAOTingru，YANG ttenanTumor Institute，Zhengzhou450008，China ABSTRACT To intothe ofCIKcells tumorcellsinvitro． Objectiveprobe cytotoxicity against Methods cellswereisolated from bloodofhealthdonorsFicoll—- Lymphocytes freshlyperipheral by thecellsobtstainedwereinduced andCD3mAb． densitycentrifugation，and byIFN’／，IL一2 Hypaque ofCIKcellswere cellsweredifferentiatedfromeffectcells Phenotypes anMysedbyFACS．Target by CFSE of cellsweredeterminedFACS．ResultsThe of dying．ThemortalityTarget by phenotypes CD3+was to the ofCD8+was expressedup 89．33％，whilepercentage 46．26％；withCD3+CD8+， itwas58．98％andwentto30．83％whenwithCD3+CD56+．CIKcells the dealing possesscytotoxiei— tumorcellsofNKsensitive IFN tyagainst K562、insensitiveHL一60、Hela