东北农业大学生态学专业学士学位论文.doc

东北农业大学学士学位论文 　　　　　　　　　　学号：盐胁迫下二倍体马铃薯叶绿素含量 和抗氧化酶活性的变化 学生姓名： 指导教师： 所在院系： 所学专业：生态学 研究方向：农业生态学 东 北 农 业 大 学 中国·哈尔滨 2014 年 5 月 Bachelor Dissertation of Northeast Agricultural University R N: A03xxxxxx Change of Chlorophyll Content and Antioxidant Enzyme Activity of Diploid Potato under Salt Stress Student: Zh Xx Advisor: B Ym College: Resources and Environment Speciality: Ecology Direction: Agriculture Ecology Northeast Agricultural University Harbin ? China May，2014 摘要 在离体条件下研究盐胁迫对二倍体马铃薯富利亚（Solanum phureja，PHU）和窄刀薯（S. stenotomum，STN）杂种（PHU-STN）无性系相关生理指标的影响，以期探讨盐胁迫下二倍体马铃薯耐盐性的生理表现。在45份PHU-STN中，根据其在盐胁迫下六项生长参数的表现选出5份耐盐无性系、5份中耐盐无性系、5份感盐无性系，建立耐盐程度不同的三个组，以四倍体品种Bintje为耐盐对照，Mainechip为感盐对照，用0（CK），10，20，30 mmol·L-1的NaCl进行胁迫，测定叶绿素含量、超氧化物歧化酶（Superoxide Dismutase，SOD）和过氧化物酶（Peroxidase，POD）活性的变化。结果表明，随着盐浓度的升高，耐盐组叶绿素含量呈先升高后降低的趋势，中耐盐组、感盐组则呈降低的趋势，二倍体组间差异极显著；耐盐组的SOD活性呈升高的趋势，中耐盐组、感盐组呈先降低后升高的趋势，二倍体组间差异极显著；耐盐组、中耐盐组POD活性呈先降低后升高的趋势，感盐组呈降低的趋势，二倍体组间差异极显著。因此，叶绿素含量、SOD和POD活性可以作为二倍体马铃薯耐盐性鉴定的间接指标。 关键词： 马铃薯，二倍体，盐胁迫，生理指标 Change of Chlorophyll Content and Antioxidant Enzyme Activity of Diploid Potato under Salt Stress Abstract Physiological traits were studied of diploid potato Solanum phureja (PHU) and S. stenotomum (STN) hybrid (PHU-STN) clones in vitro under salt stress in order to understand the physiological performance in salt tolerance of diploid potatoes. Three groups of five salt tolerant, five medium salt tolerant and five salt susceptible clones were selected based on their performances of six growth parameters in salt stress in 45 clones, and tetraploid variety Bintje (salt tolerant) and Mainechip (salt susceptible) were also included in this experiment. They were stressed in vitro at 0 (CK), 10, 20 and 30 mmol·L-1 salt concentrations, and chlorophyll content, Superoxide Dismutase (SOD) and Peroxidase (POD) activities were measured. With increase in salt concentration, chlorophyll content in slat tolerant group was increased, and then decreas