响应面法对大豆胰蛋白酶抑制剂粗提工艺的优化-河南农业科学.pdfVIP

※工艺技术 食品科学 2011, Vol. 32, No. 04 97 1 1, 2, 1,2 1 梁雪华 ，朱秀清 * ，郑环宇 ，张智宇 (1.东北农业大学食品学院，黑龙江 哈尔滨 150030 ；2. 国家大豆工程技术研究中心，黑龙江 哈尔滨 150030) 目的：对大豆胰蛋白酶抑制剂粗提工艺进行优化。方法：以提取液pH 值、热变性温度和硫酸氨饱和度 作为影响大豆胰蛋白酶抑制剂提取率的因素，通过单因素试验选取因素与水平，根据Box-Behnken 中心组合试验设 计原理，在单因素试验基础上采用三因素三水平响应面分析法，依据回归分析确定各工艺条件的主要影响因素，以 大豆胰蛋白酶抑制剂提取率为响应值作响应面分析。结果：胰蛋白酶抑制剂提取的最佳工艺条件为提取液pH5.1 、 热变性温度 72.8 ℃、硫酸氨饱和度 54.5% ，在此条件下，胰蛋白酶抑制剂的提取率达到 86% 。结论：采用响应 面分析法可以对大豆胰蛋白酶抑制剂粗提工艺进行优化。 大豆胰蛋白酶抑制剂；粗提；Box-Behnken 中心组合试验 Process Optimization for Trypsin Inhibitor Extraction from Defatted Soybean Meal by Response Surface Methodology 1 1,2, 1,2 1 LIANG Xue-hua ，ZHU Xiu-qing *，ZHENG Huan-yu ，ZHANG Zhi-yu (1. College of Food Science, Northeast Agricultural University, Harbin 150030, China； 2. National Research Center of Soybean Engineering and Technology, Harbin 150030, China) Abstract ：Objective: The conditions for the extraction of trypsin inhibitor from low-temperature defatted soybean meal were optimized. Methods: pH, thermal denaturation temperature and ammonium sulfate saturation were identified to be main variables that influence extraction efficiency by the one-factor-at-a-time method. Based on the principles of Box-Behnken central composite design, response surface analysis was employed to explore the effects of pairwise interactions among the three variables at three levels on extraction efficiency. The optimum conditions for extracting soybean trypsin inhibitor from low- temperature defatted soybean meal were found as follows: extraction solvent pH of 5.1, thermal denaturation at