enjsrv及ifnτ在妊娠早期蒙古绵羊生殖器官中的表达分析 word格式.docxVIP

Expression of enJSRV and IFN-τ in Reproduction Organs of Mongolian Sheep in Early PregnancyAbstractERV is derived from integration events during the evolution of ancient retroviruses into mammalian genomes over many millions of years. A distinctive feature of retroviruses is their presence as inherited elements in the germ line of most eukaryotes.These elements are transmitted through reproductive tract. The ovine genome contains 27 copies of endogenous retroviruses.The function of ERV is not clear in embryo development in early pregnancy. In this study, the technique of electrochemiluminescence was used to detect the content of progesterone in serum on pregnancy Day 2, 8, 10,12,14,16,19,25 and 30.At the same time, endometrium,cervix, fallopian tube and vaginal epithelium of Mongolian sheep were separated and total RNA was extracted from these tissues . IFN-τ and enJSRV fragment were amplificated using RT-PCR. We detected the relative expression of enJSRV and IFN-τ mRNA in different tissues through Real-time quantitative RT-PCR. The relationship of the expression of enJSRV and IFN-τ mRNA in reproductive tract with progesterone content in serum was researched.Electrochemiluminescence results showed the progesterone level was 0.4 ng·mL-1on Day 2 and rose afterwards on Day 8 of gestation, then kept at about 8.3 ng·mL-1 on Day 8-16 and decreased during Day 19-30 with the value 2.5 ng·mL-1 on Day 30. RT- PCR analyses displayed that enJSRV mRNA was expressed in the endometrium, cervix, fallopian tube and vaginal epithelium of Mongolian sheep and appeared the single strip, while IFN-τ only in the endometrium. Homology of enJSRV and IFN-τ is 94.9% and 96.3% respectively in GenBank.Real-Time PCR analyses demonstrated that enJSRV andIFN-τ mRNA were expressed speci?cally in uterine endometrium of Mongolian sheep. The mRNA expression level of enJSRV was high on Day 12-14, while lower than the former on Day 2-10 and 16-30 during pregnancy. IFN-τ mRNA was expressed on Day 12-25 o