fancd2 shrna干扰对头颈鳞癌颈淋巴结转移细胞化疗敏感性的影响和机制研究 word格式.docxVIP

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fancd2 shrna干扰对头颈鳞癌颈淋巴结转移细胞化疗敏感性的影响和机制研究 word格式.docx

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fancd2 shrna干扰对头颈鳞癌颈淋巴结转移细胞化疗敏感性的影响和机制研究 word格式

Effect of chemosensitivity and the mechanisms of FANCD2 shRNA interference in cervical lymph node metastasis cells of head and neck squamous cell carcinomaAbstract: Objective: The incidence of head and neck squamous cell carcinoma (HNSCCC) ranks sixth in systemic tumor. Because of the centralization of vital organs and plenty of lymph nodes in the head and neck, the lymph node metastasis of HNSCC restricts the effect of operation and radio-chemotherapy. At present, chemotherapy has widely used in clinical treatment of HNSCC, especially the patients of cervical lymph node metastasis. However, primary or acquired resistance to chemotherapy has decreased the therapeutic effect on the HNSCC patients with lymph node metastasis. Fanconi anemia (FA) pathway dysfunction can change the sensitivity of tumor cells to DNA cross-linking agents, and FANCD2 as a critical gene of the FA pathway may play an important role in affecting the tumor cell growth and chemosensitivity. In this experiment, by using the HNSCC lymph node metastasis HSC-4 cell lines which has stability of FANCD2 silent expression by shRNA interference, the possible role of FANCD2 in HNSCC growing and the effect of sensitivity to chemotherapy and the related mechanism by silencing FANCD2 expression in HNSCC neck lymph node metastasis cells were studied. Methods: Used the HNSCC neck lymph node metastasis HSC-4 cell line which had stably FANCD2 silencing effect by shRNA interference in earlyexperiment, experimental cells were divided into three groups: control group (wild-type HSC-4 cells without any treatment), negative control group (FANCD2-shRNA-C, transfected invalid interference sequence of FANCD2 in HSC-4 cells), experimental group (FANCD2-shRNA, containing effective interference sequence and stable silencing effect of FANCD2 in HSC-4 cells).After cell passage, puromycin selection, silencing effect of FANCD2 protein was detected in Western Blot, and the silencing efficiency was also calculated. Detected t