科技论文的阅读.pptVIP

Efficient Reading of Papers in Science and Technology 科技论文的阅读 1，typical sections of the experimental research paper Abstract Introduction Method results Discussion Title Conclusion ABSTRACT：研究动机，研究方法，主要结果，简要结论。 INTRODUCTION：介绍研究课题，陈述该课题已有的主要研究结果，解释你对课题研究的特殊贡献。 METHODS：描述所用材料，实验装置，实验方法，理论模型，计算方法。 论文的结构 RESULTS：如实汇报结果和数据。让结果和数据来表达研究结论。 DISCUSSION：解释结果和数据。提炼原理，揭示关联；提出模型，理论或分析；解释结果与原理或模型的关系。 CONCLUSION：总结阐明论文的主要理论和重要性，也可以同时点明局限性或有所保留的地方。 Thermally responsive reversed micelles for immobilization of enzymes Hong Chen1, Hong-Wei Yu, Ph.D.2, Li-Hong Liu3, Li-Shan Wang3, Chi-Bun Ching, Ph.D.2, Yi-Yan Yang, Ph.D.3* 1Department of Chemical and Molecular Engineering, National University of Singapore 2Department of Chemical and Molecular Engineering, Nanyang Technological University 3Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos,? #04-01, Singapore 138669 * To whom correspondence should be addressed: yyyang@ibn.a-star.edu.sg; Tel: 65 Fax: 65** This work was funded by the Institute of Bioengineering and Nanotechnology, Agency for Science, Technology and Research (A*STAR), Singapore and A*STAR-Science, Engineering and Research Council grant 032 101 0010 (to CBC and YYY) Keywords: Thermo-sensitivity; Reversed micelles; Enzyme immobilization; Enzyme recovery; Synthesis of chiral pharmaceutical Abstract In this study, thermally responsive alkyl end-capped poly(N-isopropylacrylamide-co-acrylic acid) amphiphilic copolymers were synthesized, and successfully utilized to fabricate reversed micelles and immobilize enzymes. TEM study showed that the reversed micelles were spherical in nature. The activity of Candida rugosa lipase immobilized in the reversed micelles for catalyzing the esterification of lauric acid and 1-propanol was analyzed in comparison with naked enzyme. Immobilized lipase provided much greater catalytic activity. In addition, the effects of pH, water content, polymer and enzyme concentration on the catalytic ac