刺猬棘刺的形态结构及发育-遗传学专业论文 word格式.docxVIP

目录1引言................................................................11.1动物毛被类型的多样性............................................11.2毛囊结构及发育..................................................31.3毛囊发育相关的分子调控..........................................61.4刺猬的组织学基础研究进展........................................82刺猬的形态学观察及组织学分析.......................................122.1材料...........................................................122.1.1实验材料.....................................................122.1.2实验试剂及器材...............................................122.2实验方法.......................................................122.2.1刺猬棘刺的形态学研究.........................................122.2.2刺猬棘刺长度、直径和密度的测量...............................132.2.3刺猬皮肤样品的固定和保存.....................................132.2.4样品的处理及切片的制备.......................................132.2.5HE染色132.3结果与分析.....................................................132.3.1棘刺的基本形态...............................................132.3.2棘刺生长的特点...............................................142.3.3刺猬皮肤以及棘刺结构的组织学分析.............................152.3.4刺猬胚胎棘刺发育的形态学分析.................................182.3.5刺猬背部和腹部皮肤发育过程中毛囊形态发生.....................192.4讨论...........................................................253刺猬棘刺角质化细胞和间充质细胞的增殖...............................263.1材料...........................................................273.1.1实验材料.....................................................273.1.2实验试剂及器材...............................................273.2实验方法.......................................................273.2.1BrdU标记273.2.2免疫组化.....................................................273.2.3碱性磷酸酶染色...............................................283.3结果与分析.....................................................283.3.1碱性磷酸酶和细胞角蛋白的表达.................................283.3.2刺猬棘刺发育过程中的细胞增殖.................................343.3.3棘刺发育过程中的干细胞定位...................................393.4讨论...........................................................404刺猬棘刺发育过程皮