缺氧对脂肪细胞代谢及内分泌功能的影响-生物化学与分子生物学专业论文 word格式.docxVIP

结论：1 缺氧使脂肪细胞脂解增加，FFA水平升高。2 缺氧使脂肪细胞内炎症因子水平升高，抗炎症因子水平下降，提示缺氧可能在炎症发生过程中起着重要作用。3 缺氧使脂肪细胞内葡萄糖消耗增加，但细胞内ATP含量减少。关键词：缺氧；3T3-L1细胞；脂质代谢；炎症因子；肥胖论文类型：A（基础研究）AbstractObjective:Obesityisconsideredasalow-gradedchronicinflammation，hypertrophyand hyperplasia of adipose tissue contributed to theadiposetissuehypoxia,but hypoxiais a potentialfactorforchronicinflammation.Thisstudyistocultivateandinducethe3T3-L1 pre-adipocytetodifferentiatedadipocyte,andthentesttheeffectoflipidmetabolismandendocrinefunctionin adipocytesby hypoxia(1%O2，5%CO2，94N2）.By a preliminarystudy ofhypoxiaonlipidmetabolismandendocrinefunctioninregulatorymechanisms,itoffersa new view of understanding for the pathogenesis of obesity.Methods:3T3-L1preadipocyteisinducedtodifferentiatedmatureadipocyte(oilredOstaining identification).Inadditiontothecontrolgroup,theotherforhypoxictreatment(1%O2，5%CO2，94N2),Real-TimePCRisusingtodetectthehormonesensitivelipase(HSL),fatty acid synthase (FASN)mRNA at different time points after hypoxia treatment. It is tested the proteinexpressionlevelatdifferenttimepointsofphosphorylationofThr172AMPK (pThr172-AMPK),hormonesensitivelipase(HSL),fattyacidsynthase(FASN), phosphorylationofSer565hormonesensitivelipase(pSer565-HSL)afterhypxiaby Western Blot.3T3-L1preadipocyteisinducedtodifferentiatedmatureadipocyte(oilredOstaining identification).Theadipocytesweretreatedwithnormoxiaorhypoxiafor24h,andthe supernatantwascollectedimmediatelyfortestingtheglycerol,FFAbySpectrophotometric method,lysed the cell and determinated ofthe intracellular ATP content.3T3-L1preadipocyteisinducedtodifferentiatedmatureadipocyte(oilredOstaining identification).ELISAmethodisusedtodeterminatethecontentofIL-6,TNFalphaand adiponectinatdifferenttimepointsbeforeandafterhypoxia,spectrophotometricmethod for the determination of glucose consumption .Results:Intheexperiment,3T3-L1preadipocyteisinducedtodifferentiatedmatureadipocyte(oil redOstainingidentification),3T3-L1isrecognizedastheinternationalcellmodeloffat celldifferenti