八十九年无电渗流作用下毛细电泳自由溶液中抗体抗原-CiteSeerX.PDF

八十九年度[無電滲流作用下毛細電泳自由溶液中抗體抗原間相互作用之動力學模式 系統]結案報告 計劃主持人 ：台大醫學院光電生物醫學中心林世明助理教授 計劃編號 ：NSC 89-2320-B-002-154-M08 執行期限 ：88/08/01 ~ 89/07/31 執行本年度研究計劃成果已經發表一篇學術論文在美國知名雜誌”Analytical Biochemistry”(S. Lin et al., Analytical Biochemistry 284, 422-426 (2000))並在此篇論文最後 Acknowledgment 裡陳述感謝國科會Grant NSC 89-2320-B-002-154-M08 因此,擬以此篇論文替代此年度之結案報告 Notes Tips Characterization of a Polyclonal Anti-hapten Antibody by Affinity Capillary Electrophoresis Shiming Lin a *, Jui-Chang Tsai a and Su-Ming Hsu b a b Center for Optoelectronic Biomedicine and Department of Pathology, National Taiwan University College of Medicine, Taipei, Taiwan *Corresponding author: Dr Shiming Lin Center for Optoelectronic Biomedicine, National Taiwan University College of Medicine , 1-1 Jen-Ai Road, Taipei, 100, Taiwan (Telephone : + 886-2-2394-9125 Facsimile : + 886-2-2395-7990 E-mail : shiming@ha.mc.ntu.edu.tw) Abbreviations used: ACE, affinity capillary electrophoresis; CE, capillary electrophoresis; pAb, polyclonal antibody; DNP, dinitrophenol. Abstract We used affinity capillary electrophoresis (ACE) to characterize a heterogeneous population of polyclonal antibody (pAb) specific to a hapten, dinitrophenol (DNP). The binding migration- shift behavior of anti-DNP pAb to DNP in electrophoretic solution was observed and the affinities of subpopulations were studied. At large DNP excess, the pAb gradually split in two peaks and finally separated at saturating DNP-concentrations. The minor peak migrates at constant time and the major peak shifts away from the other with the increasing amount of DNP. This major peak represents a subpopulation of low to moderate affinity and very few or no high af