EpicsXL型流式细胞仪快速检测临床标本中念珠菌耐药性.doc

EpicsXL型流式细胞仪快速检测临床标本中念珠菌耐药性 ? 70?ClinicalApplicationI医械临床 EpicsXL型流式细胞仪快速检测临床标本中念珠茵耐药性 崔幸琨,田艳生,杨宝财,孙莉,赵鹏飞 【摘要】目的:探讨EpicsXL型流式细胞仪进行念珠菌快速药敏试验的可行性.方法:应用EpicsXL型流式细胞仪检 测185株念殊菌对二性霉素B,氟康唑,伊曲康唑,酮康唑,5-FC等药物的耐药性,与M27一A标准化药敏试验结果 进行比较.结果:EpicsXL型流式细胞仪可以很好地鉴别死,活念珠茵.2种药敏结果相符,分别为:二性霉素90.8%, 氟康唑94.6%,伊曲康唑91.4%,酮康唑87.0%,5一FC89.2%.以M27一A药敏结果为评价标准,流式细胞术进行5种 药物敏感性试验的灵敏度,特异性分别为二性霉素B:83.8%(57/68),94.9%(111/117);氟康唑:92.5%(74/80),96.2% (101/105);伊曲康唑:86.5%(45/52),93.2%(124/133);酮康唑:90-4%(85/94),83.5%(76/91);5一FC:77.5%(31/40), 92.4%(134/145).结论:EpicsXL型流式细胞仪进行念珠菌快速药敏试验与常规药敏试验结果具有较好的一致性,且 快速,敏感. 【关键词】念珠茵属;药敏试验;流式细胞术 f中国图书资料分类号]R379.4[文献标识码】A[文章编号]1003—8868(2010)06—0070—03 ResearchofRapidSusceptibilityTestingofCandidaSpeciesbyEpicsXLFlowCytometry CUIXing—kun.TIANYan—sheng.YANGBao—cai,SUNLiZHAOPeng—fei fClinicalLaboratoryDepartment.GeneralHospitalofNorthChinaOilfield,Renqiu062552,HebeiProvince,China) AbstractObjectlveTodeveloparapidmethodforantifungalsusceptibilitytestingbyEpicsXLFlowCytometry(FCM). MethodsTheresistanceof185candidastofiveantimicrobialswasdetectedbyflowcytometry.andtheresultswere comparedwiththeM27一Amethod.1l~ultsConcordanceratesofthesusceptibilitytoAmphotericinB,Fluconazole, Itraconazole.Ketoconazoleand5一FCin185candidasdetectedbyEpicsXLandM27一Awere90.8%,94.6%,91.4%, 87.O%.89.2%respectively.IftheresultsoftheM27一Amethodwastakenasthegoldenstandard,thesensitivityand specificityforAmphoterieinBdetectedbyEpicsXLware83.8%(57/68)and94.9%(1】1/117),forFluconazolewere92.5% f74/801and96.2%(101/105)forItraconazolewere86.5%(45/521and93.2%f124/133),forKetoconazolewere90.4%f85/ 941and83.5%f76/91),andfor5一FCwere77.5%f31/40)and92.4%f134/145).C,ondugionEpicsXL,beingsimpleand sensitivecanbeusedasarapidmethodforcandidaspeciessusceptibilitytesting.【ChineseMedicaIEquipment JournaI,2010,31(6):70-71,731 Keywordseandida;antimicrobialsusceptibilitytest;floweytometry 近年来,随着骨髓移植,化疗药物的广泛应用,对真菌尤 其是念珠菌高度易感的免疫抑制患者数量迅速增多,已成为 免疫缺陷病患者的主要死因之一.因此,快速,简便的药敏试 验方法和及时,可靠的药敏试验报告对有效预防和控制真菌 感染尤为重要.流式细胞术(floweytometry,F