载虾青素海藻酸钙微球的制备及性能研究-精细化工专业论文.docx

载虾青素海藻酸钙微球的制备及性能研究PreparationandPropertiesofAstaxanthin-loadedCalciumAlginateMicroparticlesAbstractThepowerfulantioxidantandbiologicalactivitiesofastaxanthinareconferredbyitsuniquemolecularstructure，whichconsistsofhydroxyl(_oH)andketo(C=0)moietiesoneachiononering．However．applicationsofastaxanthinarerestricteddueto itsunstablestructure---the11conjugatedcarbon-carbondoublebonds．whichareparticularlysusceptibletooxidationinharshenviromentslikehightemperature，acidicenvironment，thepresenceoflightandoxygenetc．Consequently，thisstructuraltransformationcausesthelossofbiologicalactivity．Inaddition，astaxanthin，similartootherlipophiliccarotenoids，haslowbioavailability，resultinginlimitedinvivoantioxidantactivity．Ithasbeenreportedthatencapsulationtechnologycouldimprovethestabilityofastaxanthin，howeverthemicroparficlespreparedbytheconventionalmethodsalwayswithlOWencapsulatione伍ciencyandwidesizedistribution．IntlliSresearch，astaxanthin—loadedcalciumalginatemicroparticleswithuniformsize，gooddispersibilityandhighencapsulationefficiencywerepreparedusingatwo—emulsionsgelationmethod，aimingtoimprovethestabilityandwater-dispersionofastaxanthin,andatthesametime．itsanti．tumoractivityWaSinvestigatedinvitro．rnleoptimalemulsificationconditionWasdeterminedbyinvestigatingthestabilityofemulsion：4000r／ruinshearingrate，5minemulsifyingtime，4％(v／v)Span80concentration，theoil．waterratioof1：1．111eemulsionwasstableandwith2．0．3．0p,munifoFITIsizepreparedunderthe optimal emulsification．111e optimal condition for preparing calciumalginatemicroparticles wasdeterminedbyunivariate analysis：10％rw／v)calciumchlorideconcentration．1％sodiumalginateconcentrationandl5℃reactiontemperature．Thesizeofmicroparticlesrangedfrom0．8to3．0岬withameansizeof1．5肌andnarrowsizedistribution(PDI=0．15)．Theamountofastaxanthin，emulsion．brokingtimeandwashingtimesaffectedtheencapsulationefficiencyandastaxanthin．10adedratesignificantly．Whentheinitialamountofastaxanthinwag30mg，emulsion-brakingtimeWas30minandthewashingtimeWas1，theencapsulationemc