中华绒螯蟹短尾化发育相关基因的克隆与界定-生物学；动物学专业论文.docx

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中华绒螯蟹短尾化发育相关基因的克隆与界定-生物学；动物学专业论文.docx

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中华绒螯蟹短尾化发育相关基因的克隆与界定-生物学；动物学专业论文

CloningandcharacterizationofgenesinvolvedinthebrachyurizationdevelopmentofChinesemittencrab(Eriocheirjaponicasinensis)LIPeng(InstituteofAnimalGeneticResources,CollegeofLifeSciences,NanjingNormalUniversity,Nanjing210046,P.R.China)AbstractTheChinesemittencrab,Eriocheirjaponicasinensis(Arthropoda,Crustacea,Decapoda,Reptantia,Brachyura,Grapsidae),isoneofthemostpromisingandvaluableculturedcrabsforaquacultureinChina.Themittencrabsundergobrachyurizationmorphogenesisinearlydevelopment.Themegalopaandjuvenilecrabstagesarethefirsttoexhibitbrachyurizationandgeneralattainmentoftheadultbodyplan,yettheyaremostlyfreefromthefeaturesdevelopedlaterinadults.Thenoticeablemorphologicchangeoccursbasicallybetweenthemegalopastageandthefirstcrabstageofbrachyurancrabs,suchasabdomenreduced,flatted,andfoldedbeneaththewidenedcephalothorax,insertedbetweenthepereiopodsorinaspecialcavity,andinbothsexestheappendagesdegraded,haslostallnatatoryfunctionsandthemaleretainsonlythefirsttwopairsofpleopodsinthebrachyurizationprocess.Thebrachyurizationmorphogenesisisconservative,phylogeneticallysignificantanduniversaldevelopmentpatternofbrachyurancrabs.Uptodate,studiesonbrachyurizationmorphogenesisofbrachyurancrabsareconcentrateduponmorphology,physiologyandecology,etc.Knowledgeatthemolecularlevelofthisimportantprocess,suchasregulatorygenesinvolvedinthebrachyurizationdevelopmentofbrachyuracrabs,ispoorlyunderstoodandsurprisinglyobscure.Inthepresentstudy,differentiallyexpressedgenesbetweentheabdominaltissueofmegalopalarvaandthatofthefirstcrabstagejuvenilecrabofE.j.sinensiswereclonedandidentifiedbythesuppressionsubtractivehybridization(SSH)andexpressedsequencetags(ESTs)techniques.TwocDNAexpresslibrarieswereconstructedbySMARTtechniqueandfull-lengthcDNAofsomegenesthatmaybeinvolvedinthebrachyurizationdevelopmentwereobtainedbyRACE(rapidamplificationofcDNAends)approach.AndthenthemRNAexpressedvariabilityanalysesofsomegenesinthebrachyurizationdevelopmentprocesswereperformedbyusingsemi-quantitativeRT-PCRandfluorescentrea