胶质瘤干细胞诱导巨噬细胞癌变的实验研究-神经外科学专业论文.docx

下载文档 降价啦

3
0
约4.16万字
约 53页
2018-05-05 发布于上海
举报
版权申诉
保障服务

胶质瘤干细胞诱导巨噬细胞癌变的实验研究-神经外科学专业论文.docx

1、本文档共53页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

胶质瘤干细胞诱导巨噬细胞癌变的实验研究-神经外科学专业论文

Experimentalstudyonmalignanttransformationofmacrophages inducedbygliomastemcellsAbstrcatPartI:Experimentalstudy onmalignanttransformationofhost macrophagesinducedbygliomastemcellsinvitroObjective:Hostmacrophageswereco-culturedwithgliomastemcells.Interactions betweenhumangliomastemcellsandhostmacrophageswereobserved,especially, whethermalignanttransformationofmacrophagesinducedbygliomastemcellscould occurwasfurtherstudied,anditsbiologicalphenotypiesaftertransformationwere identified.Method:C57BL/6J-NC-GFPnudemiceexpressinggreenfluorescentprotein(GFP) wereappliedtoharvestthemacrophageswithperitoneallavage.Gliomastemcell lines(SU3)wastransfectedwithredfluorescentprotein(RFP).SU3-RFPcellswere co-culturedwithmousemacrophagestoestablishadual-colorco-cultureinvitromodel. Theinteractionsbetweenthehost macrophagesandgliomastemcellswereobserved. Morphologicalchangesofthemacrophagecellswereobserved.Identificationofthe biologicalphenotypes oftransformedmacrophages wereperformed.Results:Macrophagesexperiencedmorphologicalchangesafterinvitrostimulation bytumorstemcells. phagocytosis oftumorcellsbymacrophageswas observedintheearly stageofcellco-culture.Thenmacrophagesturnedfromsmallroundcellsintolong spindle-likecells.Cellgrowthcurveshowedthatcellcycleandthegrowthrateofthe transformedmacrophagesweresimilartothoseofSU3.Thetransformedcellshadpatial cancercellcharacteristics.Conclusion：Dual-colorfluorescenceinvitrocellco-culturemodel canbeappliedtoobserveinteractionsbetweenhumangliomastemcellsandhostmacrophages.Malignant transformationofhostmacrophagescouldbeinducedbygliomastemcellsinvitrowhich deservedfurtherstudy.KeyWords:Thedual-fluorescencetracing;Malignanttransformation;Macrophages; GliomastemcellsPartIIPreliminarystudiesonmalignanttransformationofmacrophages inducedbygliomastemcellsinvivo.Objective:Recentresearchesontumorimmunityhaveshownthattumorinfiltrating macrophages playedpromotingrolesfavoredfortumorgrowthwhentumordevelopedinto theadvancedstage,thispaperaimstofurtherexplo