大蒜总皂苷提取及含量测定-第三军医大学学报.DOC

大蒜总皂苷提取和含量测定方法的研究 罗红 陈立 高钰琪 第三军医大学高原医学系病理生理学教研室 摘　要：70％乙醇（用量3L/kg蒜粉）、浸泡72小时、提取1次为最优提取条件，人参皂苷对照品在1.67μg/ml～13.33μg/ml范围内与吸光度呈良好线性关系, 回归方程为: A = 0.0138×C - 0.0108( R =0.9995)，平均回收率9.0%,RSD=2.35%。结论 该总皂苷测定方法简单，准确性、稳定性、重复性和重现性良好，说明该测定方法可行，同时根据正交设计优选的提取条件可以作为生产工艺参考。 关键词分类号：　 文献标识码：Determination of extraction technology and content of total saponins in Garlic Luohong, Chenli Gao-yuqi (Department o f pathophysiology，college of High Altitude Medicine, Third Military Medical University, Chongqing 400038, China.) Objective To develop a method for content determination and extraction of total saponins in garli. Method ginsenoside Re was used as reference substance, and vanillin-perchloric acid was applied to dertermine the content of saponins at 544 nm. The total saponins was isolated by alcohol-extracting and purified with D101 macroporous resin. The optimum alcohol-extracting factors were selected by using L9(3 4) orthogonal design. And Silica gel thin-layer chromatography was used to analyse the composition of the total saponins. Result The optimum alcohol-extracting method is to soak 1Kg garlic powder in 3L 70% alcohol for 72h. The calibration curve of ginsenoside Re showed good linearity in the range of 1.67μg/ml～13.33μg/ml, and the regression equation was A = 0.0138×C - 0.0108, R =0.9995, the average recovery of control compound is 98%, RSD=2.35%. Conclusion The determination method is simple, rapid, sensitive and accurate, which can be used for evaluating the extraction quality of total saponins. key words： total saponins orthogonal design ginsenoside Re Colorimetry 大蒜是不可或缺的饮食佐料，同时大量实验证明，它不但有抗菌、消炎、杀虫等作用，也有降血脂、降血压、抗风湿、抗肿瘤、调节机体免疫力和抗氧化等多种重要生理功效[1、2、3、.4]。 大蒜成分复杂，目前认为其活性成分主要是含硫有机化合物和皂苷类。大蒜中已经、 收稿日期： 作者简介(1970-),女,博士研究生,主要从事Tel: (023) E-mail:rona764@ 作者单位：Synergy HT多功能酶标仪（基因有限公司） R-205型旋转蒸发器（BüCHI）、J-30I冷冻离心机（美国贝克曼）、HLC-1型高效液相紫外监测仪（上海金达升华仪器有限公司）、FB-2型恒流泵（上海金达升华仪器有限公司） 等。 方法与结果 1.硅胶薄层色谱层析 1.1大蒜总皂苷的制备 蒜粉用5～6倍体积的60％乙醇浸提48小时×2（充分搅拌均匀），离心取上清，旋转蒸发去乙醇后，样品水溶