大田基黄中槲皮素和黄总黄酮的提取工艺及含量测定.docVIP

大田基黄中槲皮素和黄总黄酮的提取工艺及含量测定 【摘要】 目的: 优选大田基黄总黄酮的最佳提取工艺和建立以高效液相色谱法测定大田基黄中槲皮素含量的方法。方法: 以总黄酮含量为考察指标,采用正交设计的方法研究提取大田基黄总黄酮的工艺条件。HPLC法测定槲皮素含量μm);流动相: 甲醇-0.20%冰醋酸水溶液(42:58),流量:1.0mL/min;检测波长360nm;柱温:25℃;进样量:20μL。结果：乙醇浓度60%,温度60℃,料液比1：30,提取时间1小时,提取2次,大田基黄总黄酮得率可达38.2%，在HPLC法测定槲皮素含量进样量在20μ色谱峰的峰面积呈良好的线性关系(r=0.9), 槲皮素平均加样回收率为采用此工艺提取效果较好且操作简单迅速HPLC法测定槲皮素含量操作简便、快速,有良好的精密度和准确性,可用于的质量控制。The Extraction Technology for Total Flavonoids and Determination of quercetin by HPLC from Lysimachia fortunei Maxim [Abstract] Objective: To study an extraction Technology for Total Flavonoids and determination of quercetin by HPLC from Lysimachia fortunei Maxim. Methods: The extraction conditions of total flavones were studied through the content of total flavones extracted.HPLC determination of quartering content, for the column ZORBAX SB-C18 (250mm × 4.6mm, 5μm) ; Mobile phase: methanol -0.20% glacial acetic acid solution (42:58), flow: 1.0mL/min; detection wavelength 360nm; column temperature: 25 ℃; injection volume: 20μL .Results: 60% ethanol concentration, temperature 60 ℃, liquid than 1:30, extraction time 1 hour, 2 extract, Big yellow Hypericum japonicum total falconoid yield up to 38.2 percent. HPLC determination in quartering content when the sary rate was 103％.Conclusion: This extraction process using effective and easy to omple volume 20μL in the peak time of peak area was a good linear relationship (r = 0.984), quartering average recoveperate quickly. The HPLC determination of quartering content is simple, fast, with good precision and accuracy can be used for Lysimachia fortunei Maxim quartering in the quality control [Key Words] Lysimachia fortunei Maxim; Total Falconoid; extraction technology; HPLC; Quartering 大田基黄，本品始载《植物名实图考》，原名红丝毛根。全株无毛。根茎横走，紫红色全草含摁贝素，紫金牛醌槲皮素-3-鼠李糖基(1→2)半乳糖甙，异鼠李素-3-刺槐二糖甙，毛里求斯排草素及两种新黄酮醇甙，即槲皮素-3-(2，6-二吡喃鼠李糖基吡喃哺半乳糖甙)和异鼠李素-3-(2，6-二吡喃鼠李糖基吡喃半乳糖甙[1],有降低血脂、保肝、抗炎、扩冠、抗菌等功效,其应用开发具有广阔的前景，而对大田基黄尚缺乏系统的研究,尤其对大田基黄中黄酮类化合物的提取工艺研究报道很少。文章以乙醇为溶剂,以大田基黄为原料,通过正交试验考察了提取时间、提取温度、提取次数、提取剂中乙醇含量(