农杆菌介导转基因植物TDNA整合方式.pdfVIP

HEREDITAS (Beijing) 2011 12 , 33(12): 1327―1334 ISSN 0253-9772 综 述 DOI: 10.3724/SP.J.1005.2011.01327 农杆菌介导转基因植物T-DNA 的整合方式 杨琳, 付凤玲, 李晚忱 , 611130 农杆菌介导的遗传转化已被广泛应用于植物转基因研究。作为外源基因的载体, 农杆菌 T-DNA 片段在 植物基因组中的整合方式, 不仅影响外源基因的整合效率及稳定性, 还会影响外源基因的表达特性。文章就农 杆菌介导的T-DNA 整合的两种主要模式、规律及相关研究手段进行综述, 为农杆菌介导的转基因及T-DNA 插 入突变等相关研究提供借鉴。 农杆菌; T-DNA; 侧翼序列; 整合; 转基因 T-DNA integration patterns in transgenic plants mediated by Agrobacterium tumefaciens YANG Lin, FU Feng-Ling, LI Wan-Chen Maize Research Institute , Sichuan Agricultural University, Chengdu 611130, China Abstract: The genetic transformation mediated by Agrobacterium tumefaciens has been widely applied to research of transgenic plants. As the vector of the exotic genes, the integration patterns of T-DNA fragments affects not only transfor- mation efficiency and stability, but also expression properties of the transgenes. This review summaries the two major pat- terns and the rules of T-DNA integration in Agrobacterim-mediated transformation, rules of T-DNA mediated by Agrobac- terium tumefaciens, as well as research tools for flanking sequence amplification. It is attempted to provide references for researches on transformation and T-DNA integration mutation mediated by Agrobacterium tumefaciens . Keywords: Agrobacterium tumefaciens ; transfer DNA; flanking sequence; integration; transgene , DNA DNA, , DNA, TiRi, T-DNA。 , , T- D N A , [1,2] , 收稿日期: 2011−03−30; 修回日期: 2011−07−25 基金项目: (2009CB118400)(309