epas1 vegf在子宫内膜癌中的表达及其临床意义word格式论文.docx

中文摘要EPAS1、VEGF在子宫内膜癌中的表达及其临床意义在子宫内膜癌的发生发展中起重要作用。关键词：缺诱导因子2；血管内皮生长因子；子宫内膜癌；肿瘤缺氧；新生血管化作者：傅晓红指导教师：陈友国EPAS1、VEGF在子宫内膜癌中的表达及其临床意义英文摘要TheexpressionofendotheliaPASdomainprotein1andvascularendothelialgrowthfactoraswellastheclinicalsignificanceinendometrialcarcinomaAbstractPartⅠTheEPAS1andVEGFexpressioninendometrialcarcinomaObjectiveToinvestigatetheexpressionsofendotheliaPASdomainprotein1(EPAS1),vascularendothelialgrowthfactor(VEGF)andtherelationshipsbetweentheminhumanendometrialcarcinoma.MethodsIn46casesofresectedspecimenofendometrialcarcinomaandthecorresponding46casesofnormalendometrialtissue,RT-PCRwasusedtodetecttheexpressionlevelsofEPAS1andVEGFmRNAs,westernblottingwasusedtoexaminetheexpressionlevelsofEPAS1andVEGFproteinsrespectively．ResultsHigherexpressionsofEPAS1andVEGFweredetectedinendometrialcarcinomathaninnormalendometrialtissue,forEPAS1bothatmRNAandproteinlevel(t=4.5871,p=0.0048;t=19.4877,p=0.0001),thesameasthemRNAandproteinlevelofVEGF(t=7.0495,p=0.0001;t=98.4145,p=0.0001).ConclusionsBothEPAS1andVEGFwereoverexpressedinendometrialcarcinoma.PartⅡTheclinicalsignificanceofEPAS1andVEGFexpressioninendometrialcarcinomaObjectiveToinvestigatetheexpressionsofEPAS1,VEGFandmicrovesseldensity(MVD)aswellastherelationshipsamongthem,andtherelationshipstotheclinicopathologicfeaturesofhumanendometrialcarcinoma．MethodsIn46casesofresectedspecimenofendometrialcarcinomaandthecorresponding46casesofnormalendometrialtissue,immunohistochemicalSPmethodwasusedtoexaminetheexpressionlevelsanddistributionsofEPAS1andVEGFproteinsrespectively.MVDregardedasamarkerofangiogenesiswasexploredalso.Thecorrelationsamongthemandtheirrelationshipstotheclinicopathologiccharacteristicsofhumanendometrialcarcinomawereanalyzed．ResultsHigherexpressionsofEPAS1,VEGFandMVDweredetectedin英文摘要EPAS1、VEGF在子宫内膜癌中的表达及其临床意义endometrialcarcinomathaninnormalendometrialtissue.Furthermore,significantcorrelationswereobservedamongthem(betweenEPAS1andVEGF,r=0.7358,P=0.0041;betweenVEGFandMVD,r=0.8871,P=0.0001;betweenEPAS1andMVD,r=0.736