一个转录因子Oct4可以将滋养外胚层来源的滋养层干细胞重编程Reprogramming of Trophoblast Stem Cells into.pdf

STEM CELLS® EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS Reprogramming of Trophoblast Stem Cells into Pluripotent Stem Cells by Oct4 1,2 2 2 2 2 2 2 Tong Wu , Haitao Wang , Jing He , Lan Kang , Yonghua Jiang , Jinchao Liu , Yu Zhang , Zhaohui 2 3 3 2* Kou , Lijun Liu , Xuehong Zhang and Shaorong Gao 2 1 College of Biological Sciences, China Agricultural University, Beijing 100094, P.R. China.; National Institute of 3 Biological Sciences, NIBS, Beijing 102206, P.R. China.; Center of Reproductive Medicine Research; the First Hospital of Lanzhou University; Gansu; P.R. China. Key words. Trophoblast stem cells Pluripotent stem cells Reprogramming Cell fate conversion ABSTRACT Embryonic stem (ES) cells and trophoblast stem (TS) modifications and hypomethylation of promoter cells are both derived from early embryos, yet these regions in Oct4 and Nanog genes. Meanwhile, cells have distinct differentiation properties. ES cells methylation of promoter region in the Elf5 gene can differentiate into all three germ layer cell types, occurred during reprogramming of TS cells. The gene whereas TS cells can only differentiate into placental expression profile of OiPS cells was very similar to ES cells. It has not been determined whether TS cells can cells. Moreover, OiPS cells can differentiate into the be converted into ES-like pluripotent stem (PS) cells. three germ layer cell types in vitro and in vivo. More