Anal Chem part2-2009 化学专业英语 知识介绍.ppt

The analytes interacting most strongly with the stationary phase will take longer to pass through the system than those with weaker interactions. These interactions are usually chemical in nature, but in some cases physical interactions can also be used. chromatography can be classified based on the type of mobile phase, stationary phase and support material Types of Chromatography The primary division of chromatographic techniques is based on the type of mobile phase used in the system: Type of Chromatography Type of Mobile Phase Gas chromatography (GC) gas Liquid chromatograph (LC) liquid Name of GC Method Type of Stationary Phase Gas-solid chromatography solid, underivatized support Gas-liquid chromatography liquid-coated support Bonded-phase gas chromatography chemically-derivatized support Gas Chromatography Liquid Chromatography Name of LC Method Type of Stationary Phase Adsorption chromatography solid, underivatized support Partition chromatography liquid-coated or derivatized support Ion-exchange chromatography support containing fixed charges Size exclusion chromatography porous support Affinity chromatography support with immobilized ligand Chromatographic techniques may also be classified based on the type of support material used in the system: Packed bed (column) chromatography Open tubular (capillary) chromatography Open bed (planar) chromatography Theory of Chromatography Typical response obtained by chromatography (i.e., a chromatogram): chromatogram - concentration versus elution time tR = retention time tM = void time Wb = baseline width of the peak in time units Wh = half-height width of the peak in time units The separation of solutes in chromatography depends on two factors: (a) a difference in the retention of solutes (i.e., a difference in their time or volume of elution (b) a sufficiently narrow width of the solute peaks (i.e, good efficiency for the separation system) Solu