大豆异黄酮对去卵巢大鼠阴道上皮Bcl-2mRNA影响.docVIP

大豆异黄酮对去卵巢大鼠阴道上皮Bcl-2mRNA影响 【摘要】目的探讨大豆异黄酮对去卵巢大鼠阴道上皮细胞凋亡及Bcl-2 mRNA表达的影响。方法Wistar大鼠50只，随机分为假手术组、模型组、雌二醇组、大豆异黄酮高剂量组及低剂量组。采用原位末端标记法及原位杂交方法检测阴道上皮细胞凋亡及Bcl-2 mRNA，并应用图像分析系统进行测定。结果雌二醇组、大豆异黄酮高、低剂量组均可使去卵巢大鼠阴道重量增加，分别为(0.125±0.024)，(0.123±0.020)，(0.109±0.022) g,与模型组(0.074±0.046) g比较,差异均有统计学意义(P0.01)；假手术组未见凋亡细胞，其余各组均可见凋亡细胞，大豆异黄酮高、低剂量组凋亡细胞数与雌二醇组比较,差异均有统计学意义(P0.05)；雌二醇组、大豆异黄酮高、低剂量组的Bcl-2 mRNA平均灰度值与模型组比较,差异均有统计学意义(P0.05)，但大豆异黄酮高、低剂量组的Bcl-2 mRNA平均灰度值与雌二醇组比较，差异均无统计学意义。结论大豆异黄酮可以抑制阴道上皮细胞的凋亡，通过上调Bcl-2 mRNA的表达是其作用途径之一。 【关键词】 去卵巢大鼠；大豆异黄酮；Bcl-2 mRNA；阴道上皮细胞Effect of soy isoflavone on Bcl2 mRNA expression in vagina of ovariectomy rats SUN Xiaofang,DUAN Fei.School of Medicine,Hebei University(Baoding 071000,China) Abstract： Objective To study the effect of soy isoflavon on cell apoptosis and Bcl2 mRNA expression in the vagina of ovariectomy rats.Methods Fifty adult female Wistar rats were divided into 5 groups:shamoperated group,model group,estradiol group,high soy isoflavone group and soy isoflavone group.TURNEL staining and in situ hybridization with image analysis system were applied to detect vaginal epithelial cell apoptosis and Bcl2mRNA.Results The number of apoptotic vaginal epithelial cells in rats of estradiol and high,lowdose soy isoflavone group significantly reduced(P0.01),and the Bcl2mRNA average gray value increased(P0.05).Compared with the estradiol group,the number of apoptic cells in high and low dose soy isoflavones was significantly higher(P0.05),while the Bcl2mRNA was not different between the estradiol group and the soy isoflavone groups.Conclusion Soy isoflavones may inhibit the apoptosis of vaginal epithelial cells through increased expression of Bcl2mRNA. Key words：ovariectomy rats;soy isoflavone;Bcl-2mRNA;vaginal epithelia cell 女性绝经以后，由于卵巢功能停止，激素代谢紊乱，易患绝经期综合征。目前，临床上多采用激素替代疗法进行治疗，但此疗法可出现不规则的阴道出血，增加患子宫内膜癌及乳腺癌的潜在危险，从而使其应用受到限制。大豆异黄酮结构与雌激素相似，为杂环酚类化合物，其在具有雌激素作用的同时，还具有抗雌激素作用，可以减少子宫内膜癌的发生机率。本研究通过比较观察大豆异黄酮和雌二醇对去卵巢大鼠阴道上皮细胞凋亡及凋亡调控基因Bcl-2 mRNA表达的影响，探讨大豆异黄酮的作用机制，为实际应用