响应面法优化鲨鱼硫酸软骨素的提取条件.pdfVIP

※工艺技术 食品科学 2009, Vol. 30, No. 22 231 响应面法优化鲨鱼硫酸软骨素的提取条件 张 弘，谢果凰，茅大振，何斌辉，傅春燕，刘冰冰，杨文鸽* (宁波大学生命科学与生物工程学院，应用海洋生物技术教育部重点实验室，浙江 宁波 3 152 11) 摘 要：采用响应面分析法，研究不同提取条件(温度、时间、碱浓度) 对鲨鱼硫酸软骨素(Ch S) 得率的影响，建 立提取条件与得率之间的数学模型，并进一步研究不同的超声波处理时间对硫酸软骨素提取率和纯度的影响，确定 适宜的硫酸软骨素提取条件。结果：1)采用响应面分析法建立提取条件与硫酸软骨素得率的数学模型为Ch S(%) ＝ 14 .56 －0.34x2 －0.34x 1x2 －0.82x 12 －1.45x22 －0.47x32 ，其中碱提温度对硫酸软骨素得率影响最为显著，其次为碱 液浓度；2)通过响应面交互作用分析与优化，鲨鱼硫酸软骨素适宜的碱提条件为碱液浓度4 % 、碱提温度35 ℃、碱 提时间3h ，Ch S 得率为14 .50% ；3)在优化的碱提条件处理之前利用超声波处理20min ，硫酸软骨素得率可提高到 18 .0 7 % ，纯度为9 1.5 8% 。 关键词：鲨鱼鳍骨；硫酸软骨素；碱提；响应面法；超声波 Use of Response Surface Analysis for Optimization of Chondroitin Sulfate Extraction from Shark Fin Cartilage ZHANG Hong ，XIE Guo-huang ，MA O Da-zhen ，HE Bin -hu i ，FU Chun-yan ，LIU Bin g-bing ，YANG Wen-ge* (Faculty of Life Science and Biotechnology , Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 3 152 11, China) Abstract ：Chondroitin sulfate (Ch S) was extracted from shark fin cartilage, and the effects of alkaline concentration (x 1), extraction temperature (x2) and time (x3) on the yield were studied. By conducting response surface analysis, the optimum extraction conditions were obtained, and a multiple regression model was set up between the extraction condition s and the yield of ChS, then the effect of ultrasonic time on the yield and purity of ChS was studied. The mathematical model between the extraction conditions and yield of shark ChS as follows: ChS(%)= 14.56 －0.34x2 －0.34x 1x2 －0.82x 12 －1.45x22 －0.47x32 . The extraction temperature significantly affected the yield of ChS, followed by the alkaline concentration. According to the response surface analysis, the optimal extraction conditions were as