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DMD-based LED-illumination Super-resolution and optical sectioning SUBJECT AREAS: SUPER-RESOLUTION microscopy MICROSCOPY CELLULAR IMAGING 1 1 1 2 3 3 1 Dan Dan , Ming Lei , Baoli Yao , Wen Wang , Martin Winterhalder , Andreas Zumbusch , Yujiao Qi , Liang Xia2 1 1 1 1 1 TECHNIQUES AND , Shaohui Yan , Yanlong Yang , Peng Gao , Tong Ye Wei Zhao INSTRUMENTATION OPTICAL TECHNIQUES 1 State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an 710119, China, 2Department of Anatomy K. K. Leung Brain Research Centre, Fourth Military Medical University, Xi’an 710032, China, 3Department of Chemistry, University of Konstanz, D-78457 Konstanz, Germany. Received 14 November 2012 Accepted Super-resolution three-dimensional (3D) optical microscopy has incomparable advantages over other 18 December 2012 high-resolution microscopic technologies, such as electron microscopy and atomic force microscopy, in the study of biological molecules, pathways and events in live cells and tissues. We present a novel approach of Published structured illumination microscopy (SIM) by using a digital micromirror device (DMD) for fringe 23 January 2013 projection and a low-coherence LED light for illumination. The lateral resolution of 90 nm and the optical sectioning depth of 120 mm were ach